Dexamethasone inhibits spontaneous apoptosis in primary cultures of human and rat hepatocytes via Bcl-2 and Bcl-xL induction
- PMID: 11319611
- DOI: 10.1038/sj.cdd.4400815
Dexamethasone inhibits spontaneous apoptosis in primary cultures of human and rat hepatocytes via Bcl-2 and Bcl-xL induction
Abstract
We examined the effects of dexamethasone (DEX) on the apoptotic process in primary cultures of human and rat hepatocytes. DEX prolonged cell viability, inhibited the development of an apoptotic morphology, and stabilised the expression of procaspase-3 in both human and rat hepatocytes. In addition, the inhibition of apoptosis by DEX was strongly correlated with a decrease of caspase-3-like protease activity. Moreover, DEX treatment increased the expression of anti-apoptotic Bcl-2 and Bcl-xL proteins in human and rat hepatocytes, respectively, whereas the expression of pro-apoptotic proteins Bcl-xS or Bad was not detected or remained unchanged. The bcl-xL transcript is regulated at the transcriptional level and its expression paralleled that of Bcl-xL protein in DEX-treated rat hepatocytes. Taken together, these results indicate that this glucocorticoid exerts a protective role on cell survival and it delays apoptosis of human and rat hepatocytes by modulating caspase-3-like protease activity and bcl-2 and bcl-x gene expression.
Similar articles
-
Inhibition by dexamethasone of transforming growth factor beta1-induced apoptosis in rat hepatoma cells: a possible association with Bcl-xL induction.Hepatology. 1998 Apr;27(4):959-66. doi: 10.1002/hep.510270410. Hepatology. 1998. PMID: 9537434
-
Dexamethasone protects primary cultured hepatocytes from death receptor-mediated apoptosis by upregulation of cFLIP.Cell Death Differ. 2006 Mar;13(3):512-23. doi: 10.1038/sj.cdd.4401771. Cell Death Differ. 2006. PMID: 16167066
-
Dexamethasone inhibits apoptosis in C6 glioma cells through increased expression of Bcl-XL.Apoptosis. 2006 Jul;11(7):1247-55. doi: 10.1007/s10495-006-7233-1. Apoptosis. 2006. PMID: 16699951
-
Regulation of bcl-2, bcl-XL and bax in the control of apoptosis by hematopoietic cytokines and dexamethasone.Cell Growth Differ. 1995 Jun;6(6):647-53. Cell Growth Differ. 1995. PMID: 7669718
-
[Messenger RNA for Cell Editing -Disease Treatment and Cell Transplantation Using Pro-survival mRNA].Yakugaku Zasshi. 2021;141(5):655-659. doi: 10.1248/yakushi.20-00219-5. Yakugaku Zasshi. 2021. PMID: 33952747 Review. Japanese.
Cited by
-
Comparison of the induction profile for drug disposition proteins by typical nuclear receptor activators in human hepatic and intestinal cells.Br J Pharmacol. 2008 Feb;153(4):805-19. doi: 10.1038/sj.bjp.0707601. Epub 2007 Nov 26. Br J Pharmacol. 2008. PMID: 18037906 Free PMC article.
-
Primary hepatocytes and their cultures in liver apoptosis research.Arch Toxicol. 2014 Feb;88(2):199-212. doi: 10.1007/s00204-013-1123-4. Epub 2013 Sep 8. Arch Toxicol. 2014. PMID: 24013573 Free PMC article. Review.
-
Methylprednisolone protects oligodendrocytes but not neurons after spinal cord injury.J Neurosci. 2008 Mar 19;28(12):3141-9. doi: 10.1523/JNEUROSCI.5547-07.2008. J Neurosci. 2008. PMID: 18354017 Free PMC article.
-
Research Resources for Nuclear Receptor Signaling Pathways.Mol Pharmacol. 2016 Aug;90(2):153-9. doi: 10.1124/mol.116.103713. Epub 2016 May 23. Mol Pharmacol. 2016. PMID: 27216565 Free PMC article. Review.
-
Phosphatidylinositol 3-Akt-kinase-dependent phosphorylation of p21(Waf1/Cip1) as a novel mechanism of neuroprotection by glucocorticoids.J Neurosci. 2007 Apr 25;27(17):4562-71. doi: 10.1523/JNEUROSCI.5110-06.2007. J Neurosci. 2007. PMID: 17460069 Free PMC article.
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Research Materials