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. 2001 Mar;8(3):271-9.
doi: 10.1038/85029.

Structures of two natural product methyltransferases reveal the basis for substrate specificity in plant O-methyltransferases

C Zubieta  1 , X Z He, R A Dixon, J P Noel
Affiliations

Structures of two natural product methyltransferases reveal the basis for substrate specificity in plant O-methyltransferases

C Zubieta et al. Nat Struct Biol. 2001 Mar.

Abstract

Chalcone O-methyltransferase (ChOMT) and isoflavone O-methyltransferase (IOMT) are S-adenosyl-l-methionine (SAM) dependent plant natural product methyltransferases involved in secondary metabolism in Medicago sativa (alfalfa). Here we report the crystal structure of ChOMT in complex with the product S-adenosyl-l-homocysteine and the substrate isoliquiritigenin (4,2',4'-trihydroxychalcone) refined to 1.8 A as well as the crystal structure of IOMT in complex with the products S-adenosyl-l-homocysteine and isoformononetin (4'-hydroxy-7-methoxyisoflavone) refined to 1.4 A. These two OMTs constitute the first plant methyltransferases to be structurally characterized and reveal a novel oligomerization domain and the molecular determinants for substrate selection. As such, this work provides a structural basis for understanding the substrate specificity of the diverse family of plant OMTs and facilitates the engineering of novel activities in this extensive class of natural product biosynthetic enzymes.

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