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. 2001 Feb 1;280(1):31-40.
doi: 10.1006/viro.2000.0731.

Intracellular targeting of walleye dermal sarcoma virus Orf A (rv-cyclin)

J Rovnak  1 , J W Casey, S L Quackenbush
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Free article

Intracellular targeting of walleye dermal sarcoma virus Orf A (rv-cyclin)

J Rovnak et al. Virology. .
Free article

Abstract

Walleye dermal sarcoma virus (WDSV) induces tumors and allows or possibly directs tumor regression. WDSV encodes a putative cyclin homologue, Orf A, and six variant Orf A transcripts have been identified. Northern analysis indicated that a 3.3-kb transcript, encoding full-length Orf A, is the predominant transcript in developing, but not regressing, tumors. Three Orf A proteins, one full-length and two amino-truncated forms, were expressed in mammalian and piscine cells, and their intracellular locations were determined. The full-length form was nuclear and concentrated in interchromatin granule clusters, defined by colocalization with SC-35. The amino-truncated forms were cytoplasmic. Fusion of amino-terminal portions of Orf A to a heterologous protein demonstrated that residues 1-112 were necessary for nuclear localization. Mutation of aa K80 and/or E110 disrupted nuclear localization, suggesting a mechanism similar to that of cellular A- and D-type cyclins for its nuclear import.

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