Human peripheral blood cells differentially recognize and respond to two distinct CPG motifs
- PMID: 11160295
- DOI: 10.4049/jimmunol.166.4.2372
Human peripheral blood cells differentially recognize and respond to two distinct CPG motifs
Abstract
Oligodeoxynucleotides (ODN) that contain unmethylated CpG dinucleotides trigger a strong innate immune response in vertebrates. CpG ODN show promise as vaccine adjuvants, anti-allergens, and immunoprotective agents in animal models. Their transition to clinical use requires the identification of motifs that are optimally stimulatory in humans. Analysis of hundreds of novel ODN resulted in the identification and characterization of two structurally distinct "clusters" of immunostimulatory CpG ODN. One cluster ("D") preferentially stimulates IFN-gamma production by NK cells, whereas the other ("K") stimulates cell proliferation and the production of IL-6 and IgM by monocytes and B cells. The distinct immunostimulatory properties of K and D ODN can improve the design of CpG-based products to achieve specific therapeutic goals.
Similar articles
-
Differential and competitive activation of human immune cells by distinct classes of CpG oligodeoxynucleotide.J Leukoc Biol. 2002 May;71(5):813-20. J Leukoc Biol. 2002. PMID: 11994506
-
Induction of interleukin-6 and interleukin-12 in bovine B lymphocytes, monocytes, and macrophages by a CpG oligodeoxynucleotide (ODN 2059) containing the GTCGTT motif.J Interferon Cytokine Res. 2001 Oct;21(10):871-81. doi: 10.1089/107999001753238123. J Interferon Cytokine Res. 2001. PMID: 11711000
-
Oligodeoxynucleotides containing CpG motifs induce low levels of TNF-alpha in human B lymphocytes: possible adjuvants for Th1 responses.J Immunol. 2001 Mar 15;166(6):3743-8. doi: 10.4049/jimmunol.166.6.3743. J Immunol. 2001. PMID: 11238615
-
Mechanisms and applications of immune stimulatory CpG oligodeoxynucleotides.Biochim Biophys Acta. 1999 Dec 10;1489(1):107-16. doi: 10.1016/s0167-4781(99)00147-5. Biochim Biophys Acta. 1999. PMID: 10807001 Review.
-
The immunobiology and clinical potential of immunostimulatory CpG oligodeoxynucleotides.J Leukoc Biol. 2000 Oct;68(4):455-63. J Leukoc Biol. 2000. PMID: 11037965 Review.
Cited by
-
TLR9 adjuvants enhance immunogenicity and protective efficacy of the SE36/AHG malaria vaccine in nonhuman primate models.Hum Vaccin Immunother. 2013 Feb;9(2):283-90. doi: 10.4161/hv.22950. Epub 2013 Jan 4. Hum Vaccin Immunother. 2013. PMID: 23291928 Free PMC article.
-
Development of a degenerated TaqMan real-time Q-PCR for detection of bacteria-free DNA in dialysis fluid.Biotechnol Appl Biochem. 2017 May;64(3):443-448. doi: 10.1002/bab.1498. Epub 2016 Dec 15. Biotechnol Appl Biochem. 2017. PMID: 27067406 Free PMC article.
-
TLR-9 activation of marginal zone B cells in lupus mice regulates immunity through increased IL-10 production.J Clin Immunol. 2005 Jan;25(1):29-40. doi: 10.1007/s10875-005-0355-6. J Clin Immunol. 2005. PMID: 15742155
-
Myeloid cell-targeted miR-146a mimic inhibits NF-κB-driven inflammation and leukemia progression in vivo.Blood. 2020 Jan 16;135(3):167-180. doi: 10.1182/blood.2019002045. Blood. 2020. PMID: 31805184 Free PMC article.
-
Immunostimulatory properties of phosphorothioate CpG DNA containing both 3'-5'- and 2'-5'-internucleotide linkages.Nucleic Acids Res. 2002 Apr 1;30(7):1613-9. doi: 10.1093/nar/30.7.1613. Nucleic Acids Res. 2002. PMID: 11917022 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
