Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2000 Nov;9(11):2161-9.
doi: 10.1110/ps.9.11.2161.

Solution structure of DinI provides insight into its mode of RecA inactivation

B E Ramirez  1 , O N Voloshin, R D Camerini-Otero, A Bax
Affiliations

Solution structure of DinI provides insight into its mode of RecA inactivation

B E Ramirez et al. Protein Sci. 2000 Nov.

Abstract

The Escherichia coli RecA protein triggers both DNA repair and mutagenesis in a process known as the SOS response. The 81-residue E. coli protein DinI inhibits activity of RecA in vivo. The solution structure of DinI has been determined by multidimensional triple resonance NMR spectroscopy, using restraints derived from two sets of residual dipolar couplings, obtained in bicelle and phage media, supplemented with J couplings and a moderate number of NOE restraints. DinI has an alpha/beta fold comprised of a three-stranded beta-sheet and two alpha-helices. The beta-sheet topology is unusual: the central strand is flanked by a parallel and an antiparallel strand and the sheet is remarkably flat. The structure of DinI shows that six negatively charged Glu and Asp residues on DinI's kinked C-terminal alpha-helix form an extended, negatively charged ridge. We propose that this ridge mimics the electrostatic character of the DNA phospodiester backbone, thereby enabling DinI to compete with single-stranded DNA for RecA binding. Biochemical data confirm that DinI is able to displace ssDNA from RecA.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

References

    1. Science. 1999 Dec 17;286(5448):2349-52 - PubMed
    1. Prog Nucleic Acid Res Mol Biol. 1999;63:311-66 - PubMed
    1. J Mol Biol. 1987 Apr 5;194(3):531-44 - PubMed
    1. Proc Natl Acad Sci U S A. 1988 Mar;85(6):1806-10 - PubMed
    1. J Mol Biol. 1991 Feb 5;217(3):517-30 - PubMed

Publication types

Associated data

LinkOut - more resources