The COUP-adjacent repressor (CAR) element participates in the tissue-specific expression of the ovalbumin gene
- PMID: 11118613
- DOI: 10.1016/s0167-4781(00)00241-4
The COUP-adjacent repressor (CAR) element participates in the tissue-specific expression of the ovalbumin gene
Abstract
The ovalbumin (Ov) gene is an excellent model for the study of tissue-specific gene regulation as it is only active in the estrogen-stimulated oviduct. Previous studies have demonstrated that the negative regulatory element (NRE) in the Ov gene 5'-flanking region is responsible for silencing the gene in oviduct in the absence of steroids. Linker scanning analysis defined an element within the NRE designated the COUP-adjacent repressor (CAR) element as a repressor of Ov gene expression. However, the role of the CAR element in non-oviduct tissues has not been addressed. Using transient transfection analysis of various Ov 5'-flanking region constructs into the estrogen-responsive chicken hepatocyte cell line LMH/2A, we demonstrate that Ov gene expression is not induced by estrogen and that an active repressor element exists in the NRE. Deletion analysis indicates that the region from -134 to -87, which includes the CAR element, mediates this repression. Mutation of the CAR element relieves repression, leading to high levels of gene expression. These data support a model where the inhibition of Ov gene expression in non-oviduct cells is a combination of the lack of essential positive factors and the presence of an active repressor, which binds to the CAR element.
Similar articles
-
Interferon regulatory factors (IRFs) repress transcription of the chicken ovalbumin gene.Gene. 2009 Jun 15;439(1-2):63-70. doi: 10.1016/j.gene.2009.03.016. Epub 2009 Mar 31. Gene. 2009. PMID: 19341784 Free PMC article.
-
COUP-TF plays a dual role in the regulation of the ovalbumin gene.Biochemistry. 2000 Jul 25;39(29):8537-45. doi: 10.1021/bi0005862. Biochemistry. 2000. PMID: 10913260
-
Multiple promoter elements including a novel repressor site modulate expression of the chick ovalbumin gene.DNA Cell Biol. 1999 Feb;18(2):147-56. doi: 10.1089/104454999315538. DNA Cell Biol. 1999. PMID: 10073574
-
Chick ovalbumin upstream promoter-transcription factors (COUP-TFs): coming of age.Endocr Rev. 1997 Apr;18(2):229-40. doi: 10.1210/edrv.18.2.0294. Endocr Rev. 1997. PMID: 9101138 Review. No abstract available.
-
Regulation of the lactose repressor.Int J Biochem. 1988;20(5):493-8. doi: 10.1016/0020-711x(88)90497-1. Int J Biochem. 1988. PMID: 3286312 Review. No abstract available.
Cited by
-
Interferon regulatory factors (IRFs) repress transcription of the chicken ovalbumin gene.Gene. 2009 Jun 15;439(1-2):63-70. doi: 10.1016/j.gene.2009.03.016. Epub 2009 Mar 31. Gene. 2009. PMID: 19341784 Free PMC article.
-
Positive and negative regulation of chicken anemia virus transcription.J Virol. 2005 Mar;79(5):2859-68. doi: 10.1128/JVI.79.5.2859-2868.2005. J Virol. 2005. PMID: 15709005 Free PMC article.
-
Study of the regulatory elements of the Ovalbumin gene promoter using CRISPR technology in chicken cells.J Biol Eng. 2023 Jul 17;17(1):46. doi: 10.1186/s13036-023-00367-3. J Biol Eng. 2023. PMID: 37461059 Free PMC article.
Publication types
MeSH terms
Substances
Grants and funding
LinkOut - more resources
Full Text Sources
Other Literature Sources
