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Clinical Trial
. 2000 Oct 1;528 Pt 1(Pt 1):221-6.
doi: 10.1111/j.1469-7793.2000.t01-1-00221.x.

Isoform-specific and exercise intensity-dependent activation of 5'-AMP-activated protein kinase in human skeletal muscle

J F Wojtaszewski  1 , P Nielsen, B F Hansen, E A Richter, B Kiens
Affiliations
Clinical Trial

Isoform-specific and exercise intensity-dependent activation of 5'-AMP-activated protein kinase in human skeletal muscle

J F Wojtaszewski et al. J Physiol. .

Abstract

1. 5'-AMP-activated protein kinase (AMPK) has been suggested to play a key role in the regulation of metabolism in skeletal muscle. AMPK is activated in treadmill-exercised and electrically stimulated rodent muscles. Whether AMPK is activated during exercise in humans is unknown. 2. We investigated the degree of activation and deactivation of alpha-isoforms of AMPK during and after exercise. Healthy human subjects performed bicycle exercise on two separate occasions at either a low ( approximately 50% maximum rate of O2 uptake (VO2,max) for 90 min) or a high ( approximately 75% VO2,max for 60 min) intensity. Biopsies from the vastus lateralis muscle were obtained before and immediately after exercise, and after 3 h of recovery. 3. We observed a 3- to 4-fold activation of the alpha2-AMPK isoform immediately after high intensity exercise, whereas no activation was observed after low intensity exercise. The activation of alpha2-AMPK was totally reversed 3 h after exercise. In contrast, alpha1-AMPK was not activated during either of the two exercise trials. 4. The in vitro AMP dependency of alpha2-AMPK was significantly greater than that of alpha1-AMPK ( approximately 3- vs. approximately 2-fold). 5. We conclude that in humans activation of alpha2-AMPK during exercise is dependent upon exercise intensity. The stable activation of alpha2-AMPK, presumably due to the activation of an upstream AMPK kinase, is compatible with a role for this kinase complex in the regulation of skeletal muscle metabolism during exercise, whereas the lack of stable alpha1-AMPK activation makes this kinase complex a less likely candidate.

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Figures

Figure 1
Figure 1
Effects of exercise on isoform-specific AMPK activity Muscle α2-AMPK (A) and α1-AMPK (B) activity in the low intensity exercise trial (top panels) and in the high intensity exercise trial (bottom panels). Activities were measured in vitro in the absence (○) or presence (•) of AMP. Significant differences (P < 0.05) from Basal as well as 3h Post Ex (*) and from the same measurement in the absence of AMP (†) are indicated. Data are presented as means ±s.e.m., n = 6–7.
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Comment in

  • How to avoid running on empty.
    Rennie MJ. Rennie MJ. J Physiol. 2000 Oct 1;528 Pt 1(Pt 1):3. doi: 10.1111/j.1469-7793.2000.t01-1-00003.x. J Physiol. 2000. PMID: 11018100 Free PMC article. No abstract available.

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References

    1. Cheung CF, Salt IP, Davies A, Hardie DG, Carling D. Characterization of AMP-activated protein kinase gamma subunit isoforms and their role in AMP binding. Biochemical Journal. 2000;346:659–669. - PMC - PubMed
    1. Dean DJ, Daugaard JR, Young ME, Saha A, Vavvas D, Asp S, Kiens B, Kim K-H, Witters LA, Richter EA, Ruderman NB. Exercise disminishes the activity of acetyl CoA carboxylase in human muscle. Diabetes. 2000;49:1295–1300. - PubMed
    1. Derave W, Hua AI, Ihleman J, Witters LA, Kristiansen S, Richter EA, Ploug T. Dissociation of AMP-activated protein kinase activation and glucose transport in contracting slow-twitch muscle. Diabetes. 2000;49:1281–1287. - PubMed
    1. Gao G, Widmer J, Stapleton D, Teh T, Cox T, Kemp BE, Witters LA. Catalytic subunits of the porcine and rat 5′-AMP-activated protein kinase are members of the SNF1 protein kinase family. Biochimica et Biophysica Acta. 1995;1266:73–82. - PubMed
    1. Hardie DG, Carling D, Carlson M. The AMP-activated/SNF1 protein kinase subfamily: metabolic sensors of the eukaryotic cell? Annual Reviews of Biochemistry. 1998;67:821–855. - PubMed

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