Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1975 Apr;15(4):913-7.
doi: 10.1128/JVI.15.4.913-917.1975.

Inhibition of group B arbovirus antigen production and replication in cells enucleated with cytochalasin B

K A Kos, B A Osborne, R A Goldsby

Inhibition of group B arbovirus antigen production and replication in cells enucleated with cytochalasin B

K A Kos et al. J Virol. 1975 Apr.

Abstract

A comparative study of the growth of Sindbis (SIN) virus, a group A arbovirus (togavirus), and Japanese encephalitis (JE) virus, a representative group B arbovirus (togavirus), was conducted in enucleate and nucleate cells. Immunofluorescent tests and yield measurements demonstrated that chicken embryo cells which had been enucleated and subsequently infected with SIN virus produced virus-specific antigens and infectious virus. By contrast, JE failed to replicate or produce virus-specific antigen in cells which had been enucleated before or even 2 h post infection. Studies of the effect of enulceation at various times after infection demonstrated that a nucleus must be present at least 2 and possibly as long as 4 h after infection to produce either JE-specific antigen or infectious JE virus. These studies demonstrate that the replication of SIN, a group A arbovirus (togavirus), which has no nuclear requirement, contrasts sharply with that of a group B arbovirus (togavirus), JE, which may have an initial dependence on a nucleus-associated process.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

References

    1. Am J Trop Med Hyg. 1959 Nov;8:651-64 - PubMed
    1. J Ultrastruct Res. 1965 Apr;12:317-27 - PubMed
    1. Lab Invest. 1968 Dec;19(6):652-62 - PubMed
    1. Am J Epidemiol. 1967 Nov;86(3):776-90 - PubMed
    1. J Virol. 1969 Apr;3(4):385-94 - PubMed

Publication types

MeSH terms

LinkOut - more resources