An RNA-dependent RNA polymerase gene in Arabidopsis is required for posttranscriptional gene silencing mediated by a transgene but not by a virus
- PMID: 10850496
- DOI: 10.1016/s0092-8674(00)80864-8
An RNA-dependent RNA polymerase gene in Arabidopsis is required for posttranscriptional gene silencing mediated by a transgene but not by a virus
Abstract
Posttranscriptional gene silencing is a defense mechanism in plants that is similar to quelling in fungi and RNA interference in animals. Here, we describe four genetic loci that are required for posttranscriptional gene silencing in Arabidopsis. One of these, SDE1, is a plant homolog of QDE-1 in Neurospora crassa that encodes an RNA-dependent RNA polymerase. The sde1 mutation was specific for posttranscriptional gene silencing induced by transgenes rather than by viruses. We propose that the role of SDE1 is to synthesize a double-stranded RNA initiator of posttranscriptional gene silencing. According to this idea, when a virus induces posttranscriptional gene silencing, the virus-encoded RNA polymerase would produce the double-stranded RNA and SDE1 would be redundant.
Similar articles
-
Gene silencing in Neurospora crassa requires a protein homologous to RNA-dependent RNA polymerase.Nature. 1999 May 13;399(6732):166-9. doi: 10.1038/20215. Nature. 1999. PMID: 10335848
-
Cellular RNA-dependent RNA polymerase involved in posttranscriptional gene silencing has two distinct activity modes.Mol Cell. 2002 Dec;10(6):1417-27. doi: 10.1016/s1097-2765(02)00780-3. Mol Cell. 2002. PMID: 12504016
-
Isolation of quelling-defective (qde) mutants impaired in posttranscriptional transgene-induced gene silencing in Neurospora crassa.Proc Natl Acad Sci U S A. 1997 Sep 16;94(19):10233-8. doi: 10.1073/pnas.94.19.10233. Proc Natl Acad Sci U S A. 1997. PMID: 9294193 Free PMC article.
-
Quelling in Neurospora crassa.Adv Genet. 2002;46:277-303. doi: 10.1016/s0065-2660(02)46010-5. Adv Genet. 2002. PMID: 11931228 Review. No abstract available.
-
A conserved mechanism for post-transcriptional gene silencing?Genome Biol. 2000;1(3):REVIEWS1018. doi: 10.1186/gb-2000-1-3-reviews1018. Epub 2000 Sep 13. Genome Biol. 2000. PMID: 11178243 Free PMC article. Review.
Cited by
-
Amplification of siRNA in Caenorhabditis elegans generates a transgenerational sequence-targeted histone H3 lysine 9 methylation footprint.Nat Genet. 2012 Jan 8;44(2):157-64. doi: 10.1038/ng.1039. Nat Genet. 2012. PMID: 22231482 Free PMC article.
-
Suppression of RNA-dependent RNA polymerase 6 in tomatoes allows potato spindle tuber viroid to invade basal part but not apical part including pluripotent stem cells of shoot apical meristem.PLoS One. 2020 Jul 27;15(7):e0236481. doi: 10.1371/journal.pone.0236481. eCollection 2020. PLoS One. 2020. PMID: 32716919 Free PMC article.
-
Antiviral RNA silencing initiated in the absence of RDE-4, a double-stranded RNA binding protein, in Caenorhabditis elegans.J Virol. 2013 Oct;87(19):10721-9. doi: 10.1128/JVI.01305-13. Epub 2013 Jul 24. J Virol. 2013. PMID: 23885080 Free PMC article.
-
OsRDR6 plays role in host defense against double-stranded RNA virus, Rice Dwarf Phytoreovirus.Sci Rep. 2015 Jul 13;5:11324. doi: 10.1038/srep11324. Sci Rep. 2015. PMID: 26165755 Free PMC article.
-
Light-dependent RNA interference with nucleobase-caged siRNAs.RNA. 2007 Dec;13(12):2341-7. doi: 10.1261/rna.753407. Epub 2007 Oct 19. RNA. 2007. PMID: 17951332 Free PMC article.
Publication types
MeSH terms
Substances
Associated data
- Actions
LinkOut - more resources
Full Text Sources
Other Literature Sources
Molecular Biology Databases
