Translating biomolecular recognition into nanomechanics
- PMID: 10764640
- DOI: 10.1126/science.288.5464.316
Translating biomolecular recognition into nanomechanics
Abstract
We report the specific transduction, via surface stress changes, of DNA hybridization and receptor-ligand binding into a direct nanomechanical response of microfabricated cantilevers. Cantilevers in an array were functionalized with a selection of biomolecules. The differential deflection of the cantilevers was found to provide a true molecular recognition signal despite large nonspecific responses of individual cantilevers. Hybridization of complementary oligonucleotides shows that a single base mismatch between two 12-mer oligonucleotides is clearly detectable. Similar experiments on protein A-immunoglobulin interactions demonstrate the wide-ranging applicability of nanomechanical transduction to detect biomolecular recognition.
Similar articles
-
Multiple label-free biodetection and quantitative DNA-binding assays on a nanomechanical cantilever array.Proc Natl Acad Sci U S A. 2002 Jul 23;99(15):9783-8. doi: 10.1073/pnas.152330199. Epub 2002 Jul 15. Proc Natl Acad Sci U S A. 2002. PMID: 12119412 Free PMC article.
-
Cantilever-based optical deflection assay for discrimination of DNA single-nucleotide mismatches.Anal Chem. 2001 Apr 1;73(7):1567-71. doi: 10.1021/ac0012748. Anal Chem. 2001. PMID: 11321310
-
Nanomechanics of the formation of DNA self-assembled monolayers and hybridization on microcantilevers.Langmuir. 2004 Oct 26;20(22):9663-8. doi: 10.1021/la0489559. Langmuir. 2004. PMID: 15491200
-
Bioassays based on molecular nanomechanics.Dis Markers. 2002;18(4):167-74. doi: 10.1155/2002/856032. Dis Markers. 2002. PMID: 12590170 Free PMC article. Review.
-
Mismatch formation in solution and on DNA microarrays: how modified nucleosides can overcome shortcomings of imperfect hybridization caused by oligonucleotide composition and base pairing.Mol Biosyst. 2008 Mar;4(3):232-45. doi: 10.1039/b713259j. Epub 2008 Feb 4. Mol Biosyst. 2008. PMID: 18437266 Review.
Cited by
-
De novo design of a stapled peptide targeting SARS-CoV-2 spike protein receptor-binding domain.RSC Med Chem. 2023 Jul 31;14(9):1722-1733. doi: 10.1039/d3md00222e. eCollection 2023 Sep 19. RSC Med Chem. 2023. PMID: 37731704 Free PMC article.
-
Biomarkers as Biomedical Bioindicators: Approaches and Techniques for the Detection, Analysis, and Validation of Novel Biomarkers of Diseases.Pharmaceutics. 2023 May 31;15(6):1630. doi: 10.3390/pharmaceutics15061630. Pharmaceutics. 2023. PMID: 37376078 Free PMC article. Review.
-
A monolithically integrated microcantilever biosensor based on partially depleted SOI CMOS technology.Microsyst Nanoeng. 2023 May 16;9:60. doi: 10.1038/s41378-023-00534-y. eCollection 2023. Microsyst Nanoeng. 2023. PMID: 37206699 Free PMC article.
-
A Genosensor Based on the Modification of a Microcantilever: A Review.Micromachines (Basel). 2023 Feb 10;14(2):427. doi: 10.3390/mi14020427. Micromachines (Basel). 2023. PMID: 36838127 Free PMC article. Review.
-
Detection of Trace Amounts of Water in Organic Solvents by DNA-Based Nanomechanical Sensors.Biosensors (Basel). 2022 Dec 1;12(12):1103. doi: 10.3390/bios12121103. Biosensors (Basel). 2022. PMID: 36551070 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Miscellaneous
