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Comparative Study
. 2000 Apr 7;288(5463):136-40.
doi: 10.1126/science.288.5463.136.

Identification of a coordinate regulator of interleukins 4, 13, and 5 by cross-species sequence comparisons

G G Loots  1 , R M Locksley, C M Blankespoor, Z E Wang, W Miller, E M Rubin, K A Frazer
Affiliations
Comparative Study

Identification of a coordinate regulator of interleukins 4, 13, and 5 by cross-species sequence comparisons

G G Loots et al. Science. .

Abstract

Long-range regulatory elements are difficult to discover experimentally; however, they tend to be conserved among mammals, suggesting that cross-species sequence comparisons should identify them. To search for regulatory sequences, we examined about 1 megabase of orthologous human and mouse sequences for conserved noncoding elements with greater than or equal to 70% identity over at least 100 base pairs. Ninety noncoding sequences meeting these criteria were discovered, and the analysis of 15 of these elements found that about 70% were conserved across mammals. Characterization of the largest element in yeast artificial chromosome transgenic mice revealed it to be a coordinate regulator of three genes, interleukin-4, interleukin-13, and interleukin-5, spread over 120 kilobases.

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