Direct extracellular contact between integrin alpha(3)beta(1) and TM4SF protein CD151
- PMID: 10734060
- DOI: 10.1074/jbc.275.13.9230
Direct extracellular contact between integrin alpha(3)beta(1) and TM4SF protein CD151
Abstract
Previously we established that the alpha(3)beta(1) integrin shows stable, specific, and stoichiometric association with the TM4SF (tetraspannin) protein CD151. Here we used a membrane impermeable cross-linking agent to show a direct association between extracellular domains of alpha(3)beta(1) and CD151. The alpha(3)beta(1)-CD151 association site was then mapped using chimeric alpha(6)/alpha(3) integrins and CD151/NAG2 TM4SF proteins. Complex formation required an extracellular alpha(3) site (amino acids (aa) 570-705) not previously known to be involved in specific integrin contacts with other proteins and a region (aa 186-217) within the large extracellular loop of CD151. Notably, the anti-CD151 monoclonal antibody TS151r binding epitope, previously implicated in alpha(3) integrin association, was mapped to the same region of CD151 (aa 186-217). Finally, we demonstrated that both NH(2)- and COOH-terminal domains of CD151 are located on the inside of the plasma membrane, thus confirming a long suspected model of TM4SF protein topology.
Similar articles
-
Transmembrane-4 superfamily proteins associate with activated protein kinase C (PKC) and link PKC to specific beta(1) integrins.J Biol Chem. 2001 Jul 6;276(27):25005-13. doi: 10.1074/jbc.M102156200. Epub 2001 Apr 26. J Biol Chem. 2001. PMID: 11325968
-
An extracellular site on tetraspanin CD151 determines alpha 3 and alpha 6 integrin-dependent cellular morphology.J Cell Biol. 2002 Sep 30;158(7):1299-309. doi: 10.1083/jcb.200204056. J Cell Biol. 2002. PMID: 12356873 Free PMC article.
-
Association of the tetraspanin CD151 with the laminin-binding integrins alpha3beta1, alpha6beta1, alpha6beta4 and alpha7beta1 in cells in culture and in vivo.J Cell Sci. 2002 Mar 15;115(Pt 6):1161-73. doi: 10.1242/jcs.115.6.1161. J Cell Sci. 2002. PMID: 11884516
-
Tetraspanin CD151 as a target for antibody-based cancer immunotherapy.Biochem Soc Trans. 2011 Apr;39(2):553-8. doi: 10.1042/BST0390553. Biochem Soc Trans. 2011. PMID: 21428938 Review.
-
Molecular Basis of Laminin-Integrin Interactions.Curr Top Membr. 2015;76:197-229. doi: 10.1016/bs.ctm.2015.07.002. Epub 2015 Aug 10. Curr Top Membr. 2015. PMID: 26610915 Review.
Cited by
-
MAL/VIP17, a new player in the regulation of NKCC2 in the kidney.Mol Biol Cell. 2010 Nov 15;21(22):3985-97. doi: 10.1091/mbc.E10-05-0456. Epub 2010 Sep 22. Mol Biol Cell. 2010. PMID: 20861303 Free PMC article.
-
Distinct ligand binding sites in integrin alpha3beta1 regulate matrix adhesion and cell-cell contact.J Cell Biol. 2003 Oct 13;163(1):177-88. doi: 10.1083/jcb.200304065. J Cell Biol. 2003. PMID: 14557254 Free PMC article.
-
Potentiation of the ligand-binding activity of integrin alpha3beta1 via association with tetraspanin CD151.Proc Natl Acad Sci U S A. 2005 Feb 8;102(6):1939-44. doi: 10.1073/pnas.0409493102. Epub 2005 Jan 26. Proc Natl Acad Sci U S A. 2005. PMID: 15677332 Free PMC article.
-
TspanC8 Tetraspanins and A Disintegrin and Metalloprotease 10 (ADAM10) Interact via Their Extracellular Regions: EVIDENCE FOR DISTINCT BINDING MECHANISMS FOR DIFFERENT TspanC8 PROTEINS.J Biol Chem. 2016 Feb 12;291(7):3145-57. doi: 10.1074/jbc.M115.703058. Epub 2015 Dec 14. J Biol Chem. 2016. PMID: 26668317 Free PMC article.
-
Regulation of Glioblastoma Tumor-Propagating Cells by the Integrin Partner Tetraspanin CD151.Neoplasia. 2016 Mar;18(3):185-98. doi: 10.1016/j.neo.2016.02.003. Neoplasia. 2016. PMID: 26992919 Free PMC article.
Publication types
MeSH terms
Substances
Grants and funding
LinkOut - more resources
Full Text Sources
Other Literature Sources
Miscellaneous
