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. 1999 Dec;61(6):994-1000.
doi: 10.4269/ajtmh.1999.61.994.

Detection and genetic relationship of dengue virus sequences in seventeen-year-old paraffin-embedded samples from Cuba

C A Sariol  1 , J L Pelegrino, A Martinez, E Arteaga, G Kouri, M G Guzman
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Detection and genetic relationship of dengue virus sequences in seventeen-year-old paraffin-embedded samples from Cuba

C A Sariol et al. Am J Trop Med Hyg. 1999 Dec.

Abstract

This study describes the use of the reverse transcriptase-polymerase chain reaction (RT-PCR) to generate dengue 2 amplicons from paraffin-embedded autopsy tissues collected in Cuba 17 years ago. The presumptive diagnoses had been made only by clinical evolution without serologic confirmation. This study confirms once again that dengue 2 virus was directly associated with the fatal cases in children and illustrates the potential of the RT-PCR for retrospective diagnosis of dengue cases 17 years after death. A close similarity in the genomic sequences of the dengue 2 RNA detected in tissue samples from fatal cases and those dengue 2 Cuban strains that had been previously investigated confirms the appropriate genomic classification of the etiologic agent associated with the 1981 dengue hemorrhagic fever Cuban epidemic.

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