Regulation of interaction of the acetyltransferase region of p300 and the DNA-binding domain of Sp1 on and through DNA binding
- PMID: 10651903
- DOI: 10.1046/j.1365-2443.2000.00302.x
Regulation of interaction of the acetyltransferase region of p300 and the DNA-binding domain of Sp1 on and through DNA binding
Abstract
Background: The coactivator p300 acts as a transcriptional adaptor for many DNA-binding activators. The finding that p300 possesses intrinsic acetyltransferase activity which, by chemically modifying histone tails affects the nucleosomal environment and transcription, has greatly advanced our understanding of its function. Subsequent recent studies have shown that non-histone proteins are also acetylated. However, one central question which has remained unanswered is how the coactivator/acetyltransferase interacts with DNA-binding activators to modulate their actions.
Results: Here we have demonstrated physical and functional interaction between the acetyltransferase region of p300 and the DNA-binding domain (DBD) of the transcription factor Sp1. This interaction stimulates DNA binding by the DBD of Sp1, which is mediated primarily by physical interaction rather than acetylation, despite acetylation of the DBD of Sp1 by the acetyltransferase region of p300. Furthermore, DNA binding by the DBD of Sp1 inhibits both its association and acetylation by the acetyltransferase region of p300.
Conclusions: These findings suggest a new role for p300 in regulating promoter access by DNA-binding activators through multiple regulatory interactions.
Similar articles
-
Regulation of transcription factor YY1 by acetylation and deacetylation.Mol Cell Biol. 2001 Sep;21(17):5979-91. doi: 10.1128/MCB.21.17.5979-5991.2001. Mol Cell Biol. 2001. PMID: 11486036 Free PMC article.
-
Angiotensinogen gene expression is dependent on signal transducer and activator of transcription 3-mediated p300/cAMP response element binding protein-binding protein coactivator recruitment and histone acetyltransferase activity.Mol Endocrinol. 2002 Apr;16(4):824-36. doi: 10.1210/mend.16.4.0811. Mol Endocrinol. 2002. PMID: 11923478
-
Epstein-Barr virus nuclear antigen 3C and prothymosin alpha interact with the p300 transcriptional coactivator at the CH1 and CH3/HAT domains and cooperate in regulation of transcription and histone acetylation.J Virol. 2002 May;76(10):4699-708. doi: 10.1128/jvi.76.10.4699-4708.2002. J Virol. 2002. PMID: 11967287 Free PMC article.
-
Acetylation of general transcription factors by histone acetyltransferases.Curr Biol. 1997 Sep 1;7(9):689-92. doi: 10.1016/s0960-9822(06)00296-x. Curr Biol. 1997. PMID: 9285713 Review.
-
Effects of the Acetyltransferase p300 on Tumour Regulation from the Novel Perspective of Posttranslational Protein Modification.Biomolecules. 2023 Feb 22;13(3):417. doi: 10.3390/biom13030417. Biomolecules. 2023. PMID: 36979352 Free PMC article. Review.
Cited by
-
RSAT peak-motifs: motif analysis in full-size ChIP-seq datasets.Nucleic Acids Res. 2012 Feb;40(4):e31. doi: 10.1093/nar/gkr1104. Epub 2011 Dec 8. Nucleic Acids Res. 2012. PMID: 22156162 Free PMC article.
-
A critical role of Sp1 transcription factor in regulating the human Ki-67 gene expression.Tumour Biol. 2011 Apr;32(2):273-83. doi: 10.1007/s13277-010-0119-4. Epub 2010 Oct 21. Tumour Biol. 2011. PMID: 20963645
-
Control of stochastic gene expression by host factors at the HIV promoter.PLoS Pathog. 2009 Jan;5(1):e1000260. doi: 10.1371/journal.ppat.1000260. Epub 2009 Jan 9. PLoS Pathog. 2009. PMID: 19132086 Free PMC article.
-
The hTERT and hTERC telomerase gene promoters are activated by the second exon of the adenoviral protein, E1A, identifying the transcriptional corepressor CtBP as a potential repressor of both genes.Neoplasia. 2005 Jun;7(6):614-22. doi: 10.1593/neo.04766. Neoplasia. 2005. PMID: 16036112 Free PMC article.
-
Beyond HAT Adaptor: TRRAP Liaisons with Sp1-Mediated Transcription.Int J Mol Sci. 2021 Nov 18;22(22):12445. doi: 10.3390/ijms222212445. Int J Mol Sci. 2021. PMID: 34830324 Free PMC article. Review.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Miscellaneous
