doi: 10.1038/72059.
Identification and characterization of the high-affinity choline transporter
Affiliations
- PMID: 10649566
- DOI: 10.1038/72059
Item in Clipboard
Identification and characterization of the high-affinity choline transporter
Nat Neurosci.
2000 Feb.
Abstract
In cholinergic neurons, high-affinity choline uptake in presynaptic terminals is the rate-limiting step in acetylcholine synthesis. Using information provided by the Caenorhabditis elegans Genome Project, we cloned a cDNA encoding the high-affinity choline transporter from C. elegans (cho-1). We subsequently used this clone to isolate the corresponding cDNA from rat (CHT1). CHT1 is not homologous to neurotransmitter transporters, but is homologous to members of the Na+-dependent glucose transporter family. Expression of CHT1 mRNA is restricted to cholinergic neurons. The characteristics of CHT1-mediated choline uptake essentially match those of high-affinity choline uptake in rat brain synaptosomes.
Similar articles
-
Molecular cloning of a human, hemicholinium-3-sensitive choline transporter.Biochem Biophys Res Commun. 2000 Oct 5;276(3):862-7. doi: 10.1006/bbrc.2000.3561. Biochem Biophys Res Commun. 2000. PMID: 11027560
-
The Caenorhabditis elegans gene T23G5.5 encodes an antidepressant- and cocaine-sensitive dopamine transporter.Mol Pharmacol. 1998 Oct;54(4):601-9. Mol Pharmacol. 1998. PMID: 9765501
-
Reduced expression and capacity of the striatal high-affinity choline transporter in hyperdopaminergic mice.Neuroscience. 2006 Aug 11;141(1):379-89. doi: 10.1016/j.neuroscience.2006.03.055. Epub 2006 May 3. Neuroscience. 2006. PMID: 16675138
-
Molecular properties of the high-affinity choline transporter CHT1.J Biochem. 2014 Oct;156(4):181-94. doi: 10.1093/jb/mvu047. Epub 2014 Jul 29. J Biochem. 2014. PMID: 25073461 Review.
-
The "ins" and "outs" of the high-affinity choline transporter CHT1.J Neurochem. 2006 Apr;97(1):1-12. doi: 10.1111/j.1471-4159.2006.03695.x. Epub 2006 Mar 8. J Neurochem. 2006. PMID: 16524384 Review.
Cited by
-
A null mutation for the alpha3 nicotinic acetylcholine (ACh) receptor gene abolishes fast synaptic activity in sympathetic ganglia and reveals that ACh output from developing preganglionic terminals is regulated in an activity-dependent retrograde manner.J Neurosci. 2005 Sep 14;25(37):8555-66. doi: 10.1523/JNEUROSCI.1983-05.2005. J Neurosci. 2005. PMID: 16162937 Free PMC article.
-
Reduction in CHT1-mediated choline uptake in primary neurons from presenilin-1 M146V mutant knock-in mice.Brain Res. 2007 Mar 2;1135(1):12-21. doi: 10.1016/j.brainres.2006.12.005. Epub 2006 Dec 29. Brain Res. 2007. PMID: 17196556 Free PMC article.
-
Characterization of social behavior in young and middle-aged ChAT-IRES-Cre mouse.PLoS One. 2022 Aug 4;17(8):e0272141. doi: 10.1371/journal.pone.0272141. eCollection 2022. PLoS One. 2022. PMID: 35925937 Free PMC article.
-
The effect of prenatal nicotine on mRNA of central cholinergic markers and hematological parameters in rat fetuses.Int J Dev Neurosci. 2008 Aug;26(5):467-75. doi: 10.1016/j.ijdevneu.2008.02.007. Epub 2008 Mar 4. Int J Dev Neurosci. 2008. PMID: 18407449 Free PMC article.
-
Choline metabolism in malignant transformation.Nat Rev Cancer. 2011 Nov 17;11(12):835-48. doi: 10.1038/nrc3162. Nat Rev Cancer. 2011. PMID: 22089420 Free PMC article. Review.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Molecular Biology Databases
