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. 1999 Nov 25;402(6760):413-8.
doi: 10.1038/46558.

Large-scale analysis of the yeast genome by transposon tagging and gene disruption

P Ross-Macdonald  1 , P S Coelho, T Roemer, S Agarwal, A Kumar, R Jansen, K H Cheung, A Sheehan, D Symoniatis, L Umansky, M Heidtman, F K Nelson, H Iwasaki, K Hager, M Gerstein, P Miller, G S Roeder, M Snyder
Affiliations

Large-scale analysis of the yeast genome by transposon tagging and gene disruption

P Ross-Macdonald et al. Nature. .

Abstract

Economical methods by which gene function may be analysed on a genomic scale are relatively scarce. To fill this need, we have developed a transposon-tagging strategy for the genome-wide analysis of disruption phenotypes, gene expression and protein localization, and have applied this method to the large-scale analysis of gene function in the budding yeast Saccharomyces cerevisiae. Here we present the largest collection of defined yeast mutants ever generated within a single genetic background--a collection of over 11,000 strains, each carrying a transposon inserted within a region of the genome expressed during vegetative growth and/or sporulation. These insertions affect nearly 2,000 annotated genes, representing about one-third of the 6,200 predicted genes in the yeast genome. We have used this collection to determine disruption phenotypes for nearly 8,000 strains using 20 different growth conditions; the resulting data sets were clustered to identify groups of functionally related genes. We have also identified over 300 previously non-annotated open reading frames and analysed by indirect immunofluorescence over 1,300 transposon-tagged proteins. In total, our study encompasses over 260,000 data points, constituting the largest functional analysis of the yeast genome ever undertaken.

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  • What do yeast proteins do?
    Hieter P. Hieter P. Nature. 1999 Nov 25;402(6760):362-3. doi: 10.1038/46443. Nature. 1999. PMID: 10586869 No abstract available.

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