On the frequency of protein glycosylation, as deduced from analysis of the SWISS-PROT database
- PMID: 10580125
- DOI: 10.1016/s0304-4165(99)00165-8
On the frequency of protein glycosylation, as deduced from analysis of the SWISS-PROT database
Abstract
The SWISS-PROT protein sequence data bank contains at present nearly 75,000 entries, almost two thirds of which include the potential N-glycosylation consensus sequence, or sequon, NXS/T (where X can be any amino acid but proline) and thus may be glycoproteins. The number of proteins filed as glycoproteins is however considerably smaller, 7942, of which 749 have been characterized with respect to the total number of their carbohydrate units and sites of attachment of the latter to the protein, as well as the nature of the carbohydrate-peptide linking group. Of these well characterized glycoproteins, about 90% carry either N-linked carbohydrate units alone or both N- and O-linked ones, attached at 1297 N-glycosylation sites (1.9 per glycoprotein molecule) and the rest are O-glycosylated only. Since the total number of sequons in the well characterized glycoproteins is 1968, their rate of occupancy is 2/3. Assuming that the same number of N-linked units and rate of sequon occupancy occur in all sequon containing proteins and that the proportion of solely O-glycosylated proteins (ca. 10%) will also be the same as among the well characterized ones, we conclude that the majority of sequon containing proteins will be found to be glycosylated and that more than half of all proteins are glycoproteins.
Similar articles
-
The alpha1-6-fucosyltransferase gene and its biological significance.Biochim Biophys Acta. 1999 Dec 6;1473(1):9-20. doi: 10.1016/s0304-4165(99)00166-x. Biochim Biophys Acta. 1999. PMID: 10580126 Review.
-
Biases and complex patterns in the residues flanking protein N-glycosylation sites.Glycobiology. 2004 Feb;14(2):95-101. doi: 10.1093/glycob/cwh004. Epub 2003 Sep 26. Glycobiology. 2004. PMID: 14514714
-
N-linked glycosylation of rabies virus glycoprotein. Individual sequons differ in their glycosylation efficiencies and influence on cell surface expression.J Biol Chem. 1992 May 25;267(15):10690-8. J Biol Chem. 1992. PMID: 1587845
-
N-glycosylation at one rabies virus glycoprotein sequon influences N-glycan processing at a distant sequon on the same molecule.Glycobiology. 2005 Jun;15(6):655-66. doi: 10.1093/glycob/cwi046. Epub 2005 Jan 26. Glycobiology. 2005. PMID: 15677380
-
Factors affecting the expression of recombinant glycoproteins.Indian J Med Res. 2007 Jul;126(1):22-7. Indian J Med Res. 2007. PMID: 17890819 Review.
Cited by
-
Automated glycopeptide analysis--review of current state and future directions.Brief Bioinform. 2013 May;14(3):361-74. doi: 10.1093/bib/bbs045. Epub 2012 Jul 27. Brief Bioinform. 2013. PMID: 22843980 Free PMC article. Review.
-
A Critical Analysis of the FDA's Omics-Driven Pharmacodynamic Biomarkers to Establish Biosimilarity.Pharmaceuticals (Basel). 2023 Nov 2;16(11):1556. doi: 10.3390/ph16111556. Pharmaceuticals (Basel). 2023. PMID: 38004421 Free PMC article. Review.
-
Genistein and daidzein, in combination, protect cellular integrity during 7,12-dimethylbenz[a]anthracene (DMBA) induced mammary carcinogenesis in Sprague-Dawley rats.Afr J Tradit Complement Altern Med. 2011;8(2):91-7. doi: 10.4314/ajtcam.v8i2.63196. Epub 2010 Dec 30. Afr J Tradit Complement Altern Med. 2011. PMID: 22238489 Free PMC article.
-
Semi-automated identification of N-Glycopeptides by hydrophilic interaction chromatography, nano-reverse-phase LC-MS/MS, and glycan database search.J Proteome Res. 2012 Mar 2;11(3):1728-40. doi: 10.1021/pr201183w. Epub 2012 Feb 6. J Proteome Res. 2012. PMID: 22239659 Free PMC article.
-
Molecular dynamics simulations of glycoproteins using CHARMM.Methods Mol Biol. 2015;1273:407-29. doi: 10.1007/978-1-4939-2343-4_25. Methods Mol Biol. 2015. PMID: 25753723 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
