The 3D positioning of ANT2 and ANT3 genes within female X chromosome territories correlates with gene activity
- PMID: 10527626
- DOI: 10.1006/excr.1999.4635
The 3D positioning of ANT2 and ANT3 genes within female X chromosome territories correlates with gene activity
Abstract
The three-dimensional positioning of the X-chromosomal adenine nucleotide translocase genes, ANT2 and ANT3, were compared in the active and inactive X chromosome territories (Xa and Xi) of female human amniotic fluid cell nuclei. ANT2 is located in Xq24-q25 and is transcriptionally active on Xa, but inactive on Xi. ANT3 is located in the pseudoautosomal region Xp22.3 and escapes X-inactivation. Three-color fluorescence in situ hybridization, confocal laser scanning microscopy, and three-dimensional image analysis revealed that transcriptionally active ANT2 and ANT3 genes were positioned more peripheral within their chromosome territory than the inactive ANT2 gene. The position of the latter was significantly more interior in the Xi territory. Although the volumes of both X territories were similar, 3D distances between ANT2 and ANT3 were significantly smaller in Xi compared to Xa territories reflecting different territory shapes. Our data show a correlation between 3D positioning and transcriptional activity of these X-specific genes.
Copyright 1999 Academic Press.
Similar articles
-
Differences of size and shape of active and inactive X-chromosome domains in human amniotic fluid cell nuclei.Microsc Res Tech. 1993 May 1;25(1):68-77. doi: 10.1002/jemt.1070250110. Microsc Res Tech. 1993. PMID: 8353309
-
A human pseudoautosomal gene, ADP/ATP translocase, escapes X-inactivation whereas a homologue on Xq is subject to X-inactivation.Nat Genet. 1993 Jan;3(1):82-7. doi: 10.1038/ng0193-82. Nat Genet. 1993. PMID: 8490661
-
A human pseudoautosomal gene encodes the ANT3 ADP/ATP translocase and escapes X-inactivation.Genomics. 1993 Apr;16(1):26-33. doi: 10.1006/geno.1993.1135. Genomics. 1993. PMID: 8486369
-
Chromosome territories, interchromatin domain compartment, and nuclear matrix: an integrated view of the functional nuclear architecture.Crit Rev Eukaryot Gene Expr. 2000;10(2):179-212. Crit Rev Eukaryot Gene Expr. 2000. PMID: 11186332 Review.
-
[What is the specific role of ANT2 in cancer cells?].Med Sci (Paris). 2005 Feb;21(2):156-61. doi: 10.1051/medsci/2005212156. Med Sci (Paris). 2005. PMID: 15691486 Review. French.
Cited by
-
Intermingling of chromosome territories in interphase suggests role in translocations and transcription-dependent associations.PLoS Biol. 2006 May;4(5):e138. doi: 10.1371/journal.pbio.0040138. Epub 2006 Apr 25. PLoS Biol. 2006. PMID: 16623600 Free PMC article.
-
Chromosomal Rearrangements and Altered Nuclear Organization: Recent Mechanistic Models in Cancer.Cancers (Basel). 2021 Nov 22;13(22):5860. doi: 10.3390/cancers13225860. Cancers (Basel). 2021. PMID: 34831011 Free PMC article. Review.
-
Transcriptomic and nuclear architecture of immune cells after LPS activation.Chromosoma. 2011 Oct;120(5):501-20. doi: 10.1007/s00412-011-0328-7. Epub 2011 Jun 22. Chromosoma. 2011. PMID: 21695480
-
New Data on Organization and Spatial Localization of B-Chromosomes in Cell Nuclei of the Yellow-Necked Mouse Apodemus flavicollis.Cells. 2021 Jul 19;10(7):1819. doi: 10.3390/cells10071819. Cells. 2021. PMID: 34359988 Free PMC article.
-
The Xist lncRNA exploits three-dimensional genome architecture to spread across the X chromosome.Science. 2013 Aug 16;341(6147):1237973. doi: 10.1126/science.1237973. Epub 2013 Jul 4. Science. 2013. PMID: 23828888 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
