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. 1999 Sep;6(5):751-5.
doi: 10.1128/CDLI.6.5.751-755.1999.

Th1 cytokine patterns in cervical human papillomavirus infection

M Scott  1 , D P Stites, A B Moscicki
Affiliations

Th1 cytokine patterns in cervical human papillomavirus infection

M Scott et al. Clin Diagn Lab Immunol. 1999 Sep.

Abstract

The host's immune response to cervical human papillomavirus (HPV) infection is poorly understood. In a longitudinal cohort of women with cervical HPV infections, defined by PCR-based HPV DNA testing, we used exfoliated cervical cells and reverse transcription-PCR to examine the cervical mucosal mRNA expression of cytokines involved in regulating cell-mediated immunity. We identified seven HPV-positive subjects who were found to have cleared their HPV infections 4 months later. In all seven, a T-helper type 1 (Th1) cytokine pattern (expression of gamma interferon and absence of interleukin-4) preceded clearance. The more variable cytokine patterns seen in HPV-negative subjects suggest that the Th1 pattern in the women with subsequent clearance was a response to the HPV infection. This contention is supported by additional cross-sectional data showing a Th1 pattern in a majority of HPV-positive women. This study establishes a feasible means for assessing local cytokine expression in the cervical milieu and demonstrates that a Th1 cytokine response is associated with subsequent clearance of cervical HPV infection.

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Figures

FIG. 1
FIG. 1
Cytokine RT-PCR results for four cytokines from two subjects (lanes 3 and 4) and a positive (lane 1) and negative (lane 2) control for each cytokine. Gel photographs were scanned on a Hewlett-Packard flatbed scanner and composited with Adobe Photoshop 4.0; labels were added with Adobe Illustrator 8.0 on a Power Macintosh computer.
See this image and copyright information in PMC

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