Interference with annexin II has no effect on entry of human cytomegalovirus into fibroblast cells
- PMID: 10423150
- DOI: 10.1099/0022-1317-80-7-1807
Interference with annexin II has no effect on entry of human cytomegalovirus into fibroblast cells
Abstract
Annexin II has been identified as a human cytomegalovirus (HCMV)-binding protein, shown to be a component of purified virions and proposed as a cellular receptor for the virus. In addition, annexin II is capable of associating with the major HCMV envelope glycoprotein, gB (gpUL55). As one approach to examine the role of annexin II in virus entry, a high-titre polyclonal annexin II-specific antibody was produced and its effects in virus entry and cell-to-cell spread assays were tested. This anti-annexin II serum recognized virion and cell surface annexin II and annexin II-derived peptides. Recombinant annexin II, with characteristic calcium- and phospholipid-binding activities, was also examined. Pretreatment of cells, virions or both with polyclonal anti-annexin II serum, affinity-purified anti-annexin II antibodies or recombinant annexin II protein prior to infection was inconsequential to the entry of HCMV into fibroblasts. HCMV was also able to dose-dependently penetrate annexin II-deficient 293 cells. Furthermore, the spread of HCMV from cell to cell was not inhibited in the presence of polyclonal anti-annexin II antibodies or exogenous annexin II protein. These experiments do not support a direct role of annexin II in virus entry or spread. Alternative roles for the gB-annexin II interaction are proposed.
Similar articles
-
Direct interaction between human cytomegalovirus glycoprotein B and cellular annexin II.J Virol. 1997 Dec;71(12):9803-7. doi: 10.1128/JVI.71.12.9803-9807.1997. J Virol. 1997. PMID: 9371650 Free PMC article.
-
Human cytomegalovirus glycoprotein complex gH/gL/gO uses PDGFR-α as a key for entry.PLoS Pathog. 2017 Apr 12;13(4):e1006281. doi: 10.1371/journal.ppat.1006281. eCollection 2017 Apr. PLoS Pathog. 2017. PMID: 28403202 Free PMC article.
-
The N Terminus of Human Cytomegalovirus Glycoprotein O Is Important for Binding to the Cellular Receptor PDGFRα.J Virol. 2019 May 15;93(11):e00138-19. doi: 10.1128/JVI.00138-19. Print 2019 Jun 1. J Virol. 2019. PMID: 30894468 Free PMC article.
-
[Interrelationship between human cytomegalovirus infection and chemokine].Nihon Rinsho. 1998 Jan;56(1):69-74. Nihon Rinsho. 1998. PMID: 9465667 Review. Japanese.
-
[The receptor and pathways for human cytomegalovirus entry].Nihon Rinsho. 1998 Jan;56(1):44-9. Nihon Rinsho. 1998. PMID: 9465663 Review. Japanese.
Cited by
-
Mass spectrometry-based investigation of measles and mumps virus proteome.Virol J. 2018 Oct 16;15(1):160. doi: 10.1186/s12985-018-1073-9. Virol J. 2018. PMID: 30326905 Free PMC article.
-
Annexin A2 in Virus Infection.Front Microbiol. 2018 Dec 5;9:2954. doi: 10.3389/fmicb.2018.02954. eCollection 2018. Front Microbiol. 2018. PMID: 30568638 Free PMC article.
-
Virus-host protein interactions as footprints of human cytomegalovirus replication.Curr Opin Virol. 2022 Feb;52:135-147. doi: 10.1016/j.coviro.2021.11.016. Epub 2021 Dec 16. Curr Opin Virol. 2022. PMID: 34923282 Free PMC article. Review.
-
The use of microRNA by human viruses: lessons from NK cells and HCMV infection.Semin Immunopathol. 2014 Nov;36(6):659-74. doi: 10.1007/s00281-014-0447-3. Epub 2014 Sep 19. Semin Immunopathol. 2014. PMID: 25234555 Review.
-
Characterization of a panel of insertion mutants in human cytomegalovirus glycoprotein B.J Virol. 2000 Feb;74(3):1383-92. doi: 10.1128/jvi.74.3.1383-1392.2000. J Virol. 2000. PMID: 10627549 Free PMC article.
Publication types
MeSH terms
Substances
Grants and funding
LinkOut - more resources
Full Text Sources
Medical
Research Materials
