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. 1999;10(6):657-77.
doi: 10.1163/156856299x00865.

Fibronectin adsorption or/and covalent grafting on chemically modified PEEK film surfaces

O Noiset  1 , Y J Schneider, J Marchand-Brynaert
Affiliations

Fibronectin adsorption or/and covalent grafting on chemically modified PEEK film surfaces

O Noiset et al. J Biomater Sci Polym Ed. 1999.

Abstract

Poly(ether ether ketone) (PEEK) films were chemically modified, by surface wet chemistry, into PEEK-OH, PEEK-NH2, and PEEK-NCO. Fibronectin (FN) adsorption, in the presence or absence of two non-ionic surfactants, was compared onto PEEK, PEEK-OH, and PEEK-NH2 on which the protein can only be adsorbed, and onto PEEK-NCO on which FN could be covalently grafted. The amounts of FN present on the various supports were assayed by ELISA and LSC (with 125I-labeled FN). The remarkable effect of Pluronic F68 in preventing non-specific protein adhesion on the less hydrophilic surfaces was pointed out. Accordingly, a procedure could be proposed that allows minimal FN adhesion vs FN fixation on PEEK-NCO. The resulting PEEK-FN film, which immobilized 120-150 ng FN cm(-2), constitutes a new substratum for cell cultivation.

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