Adult-generated neurons in the dentate gyrus send axonal projections to field CA3 and are surrounded by synaptic vesicles
- PMID: 10205022
Adult-generated neurons in the dentate gyrus send axonal projections to field CA3 and are surrounded by synaptic vesicles
Abstract
The subgranule zone of the dentate gyrus in rats has been shown to be proliferative into adulthood and senescence. However, the connectivity of newly generated, identified neurons in the adult has not been definitively described. In the present study, 9 weeks after a series of intraperitoneal injections of 5-bromo-2'-deoxyuridine (BrdU), animals received stereotaxic iontophoretic injections of Fluoro-Gold (FG) into field CA3. Three weeks after FG injections, sections were analyzed for BrdU immunoreactivity (proliferative label), FG retrograde label, and either calbindin-D28k or synaptophysin immunohistochemistry. A large proportion (up to 44%) of BrdU-labeled cells in the dentate gyrus within regions of FG retrograde label incorporated FG. All of the doubly labeled (BrdU-FG) neurons also immunolabeled with the antibody to calbindin-D28k. Many doubly labeled (BrdU-FG) cells were also surrounded in three planes by synaptophysin immunoreactivity. We conclude that newly generated neurons in the dentate gyrus have the correct immunohistochemical profile, send appropriate axonal projections to field CA3, and are surrounded by profiles containing synaptic vesicle proteins.
Similar articles
-
Anatomical integration of newly generated dentate granule neurons following traumatic brain injury in adult rats and its association to cognitive recovery.Exp Neurol. 2007 Mar;204(1):264-72. doi: 10.1016/j.expneurol.2006.11.005. Epub 2007 Jan 2. Exp Neurol. 2007. PMID: 17198703
-
Connectional specification of regenerating entorhinal projection neuron classes cannot be overridden by altered target availability in postnatal organotypic slice co-culture.Exp Neurol. 1996 Nov;142(1):151-60. doi: 10.1006/exnr.1996.0186. Exp Neurol. 1996. PMID: 8912906
-
Granule neurons generated during development extend divergent axon collaterals to hippocampal area CA3.J Comp Neurol. 2002 Oct 28;452(4):324-33. doi: 10.1002/cne.10386. J Comp Neurol. 2002. PMID: 12355416
-
Differential calbindin-immunoreactivity in dopamine neurons projecting to the rat striatal complex.Eur J Neurosci. 2000 Dec;12(12):4578-82. Eur J Neurosci. 2000. PMID: 11122372
-
Ultrastructure and synaptic connectivity of cell types in the adult rat dentate gyrus.Prog Brain Res. 2007;163:155-66. doi: 10.1016/S0079-6123(07)63009-X. Prog Brain Res. 2007. PMID: 17765717 Review.
Cited by
-
Neuroprotective and neurorestorative effects of thymosin β4 treatment following experimental traumatic brain injury.Ann N Y Acad Sci. 2012 Oct;1270:51-8. doi: 10.1111/j.1749-6632.2012.06683.x. Ann N Y Acad Sci. 2012. PMID: 23050817 Free PMC article.
-
Comparative transduction efficiency of AAV vector serotypes 1-6 in the substantia nigra and striatum of the primate brain.Mol Ther. 2010 Mar;18(3):588-93. doi: 10.1038/mt.2009.286. Epub 2009 Dec 15. Mol Ther. 2010. PMID: 20010918 Free PMC article.
-
Injury-induced neurogenesis: consideration of resident microglia as supportive of neural progenitor cells.Neurotox Res. 2011 Feb;19(2):341-52. doi: 10.1007/s12640-010-9199-6. Epub 2010 Jun 4. Neurotox Res. 2011. PMID: 20524106 Free PMC article.
-
Intrauterine exposure to dexamethasone impairs proliferation but not neuronal differentiation in the dentate gyrus of newborn common marmoset monkeys.Brain Pathol. 2006 Jul;16(3):209-17. doi: 10.1111/j.1750-3639.2006.00021.x. Brain Pathol. 2006. PMID: 16911478 Free PMC article.
-
Learning increases the survival of newborn neurons provided that learning is difficult to achieve and successful.J Cogn Neurosci. 2011 Sep;23(9):2159-70. doi: 10.1162/jocn.2010.21597. Epub 2010 Oct 18. J Cogn Neurosci. 2011. PMID: 20954935 Free PMC article.
Publication types
MeSH terms
Substances
Grants and funding
LinkOut - more resources
Full Text Sources
Other Literature Sources
Miscellaneous
