The hookworm platelet inhibitor: functional blockade of integrins GPIIb/IIIa (alphaIIbbeta3) and GPIa/IIa (alpha2beta1) inhibits platelet aggregation and adhesion in vitro
- PMID: 10191228
- DOI: 10.1086/314724
The hookworm platelet inhibitor: functional blockade of integrins GPIIb/IIIa (alphaIIbbeta3) and GPIa/IIa (alpha2beta1) inhibits platelet aggregation and adhesion in vitro
Abstract
Hookworms, aggressive, blood-feeding, intestinal nematodes, are currently a leading cause of iron deficiency anemia in the developing world. An inhibitor of platelet aggregation and adhesion has been partially purified and characterized from soluble protein extracts of adult Ancylostoma caninum hookworms. This protein, named the hookworm platelet inhibitor, has an estimated molecular mass of 15 kDa as determined by size-exclusion chromatography. In addition to blocking platelet aggregation in response to a variety of agonists, the partially purified inhibitor also prevents adhesion of resting platelets to immobilized fibrinogen and collagen. Inhibitory monoclonal antibodies were used to identify specific blockade of cell surface integrins GPIIb/IIIa (alphaIIbbeta3) and GPIa/IIa (alpha2beta1), the platelet receptors for fibrinogen and collagen, respectively. This broad-spectrum anti-platelet activity is also present in excretory and secretory products of adult worms, suggesting a biologic role for the hookworm platelet inhibitor in vivo.
Similar articles
-
Isolation and molecular cloning of a secreted hookworm platelet inhibitor from adult Ancylostoma caninum.Mol Biochem Parasitol. 2003 Jul;129(2):167-77. doi: 10.1016/s0166-6851(03)00121-x. Mol Biochem Parasitol. 2003. PMID: 12850261
-
Principal role of glycoprotein VI in alpha2beta1 and alphaIIbbeta3 activation during collagen-induced thrombus formation.Arterioscler Thromb Vasc Biol. 2004 Sep;24(9):1727-33. doi: 10.1161/01.ATV.0000137974.85068.93. Epub 2004 Jul 1. Arterioscler Thromb Vasc Biol. 2004. PMID: 15231520
-
The parasitic hematophagous worm Haemonchus contortus inhibits human platelet aggregation and adhesion: partial purification of a platelet inhibitor.Thromb Haemost. 2002 May;87(5):899-904. Thromb Haemost. 2002. PMID: 12038795
-
Novel platelet inhibitors.Annu Rev Med. 2001;52:161-84. doi: 10.1146/annurev.med.52.1.161. Annu Rev Med. 2001. PMID: 11160773 Review.
-
Platelet integrins.Thromb Haemost. 1995 Jul;74(1):352-9. Thromb Haemost. 1995. PMID: 8578483 Review. No abstract available.
Cited by
-
The structure of hookworm platelet inhibitor (HPI), a CAP superfamily member from Ancylostoma caninum.Acta Crystallogr F Struct Biol Commun. 2015 Jun;71(Pt 6):643-9. doi: 10.1107/S2053230X1500271X. Epub 2015 May 20. Acta Crystallogr F Struct Biol Commun. 2015. PMID: 26057788 Free PMC article.
-
Twenty-five-year research progress in hookworm excretory/secretory products.Parasit Vectors. 2020 Mar 14;13(1):136. doi: 10.1186/s13071-020-04010-8. Parasit Vectors. 2020. PMID: 32171305 Free PMC article. Review.
-
Vaccination with recombinant aspartic hemoglobinase reduces parasite load and blood loss after hookworm infection in dogs.PLoS Med. 2005 Oct;2(10):e295. doi: 10.1371/journal.pmed.0020295. Epub 2005 Oct 4. PLoS Med. 2005. PMID: 16231975 Free PMC article. Clinical Trial.
-
An Update on the Geohelminths: Ascaris lumbricoides, Hookworms, Trichuris trichiura, and Strongyloides stercoralis.Curr Infect Dis Rep. 2002 Feb;4(1):59-64. doi: 10.1007/s11908-002-0068-1. Curr Infect Dis Rep. 2002. PMID: 11853658
-
Exogenous Integrin αIIbβ3 Inhibitors Revisited: Past, Present and Future Applications.Int J Mol Sci. 2021 Mar 25;22(7):3366. doi: 10.3390/ijms22073366. Int J Mol Sci. 2021. PMID: 33806083 Free PMC article. Review.
Publication types
MeSH terms
Substances
Grants and funding
LinkOut - more resources
Full Text Sources
Other Literature Sources
Medical
