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. 1999 Feb;13(2):175-80.
doi: 10.1023/a:1008301415843.

A doublet-separated sensitivity-enhanced HSQC for the determination of scalar and dipolar one-bond J-couplings

F Cordier  1 , A J Dingley, S Grzesiek
Affiliations

A doublet-separated sensitivity-enhanced HSQC for the determination of scalar and dipolar one-bond J-couplings

F Cordier et al. J Biomol NMR. 1999 Feb.

Abstract

A simple, sensitivity-enhanced HSQC experiment is described which separates the upfield and downfield components in the indirect dimension into different subspectra. The sequence is similar to the generalized TROSY scheme; however, decoupling of the X-nucleus is used during detection. A detailed analysis of relaxation effects, precision and sensitivity of the method is presented. The approach is demonstrated in a two-dimensional water flip-back 1H-15N HSQC which measures 1JHN splittings in isotropic and oriented samples of ubiquitin and the hepatitis C protease. The results are in excellent agreement with splittings obtained from a conventional 1H-coupled HSQC.

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