Beta-amyloid induces local neurite degeneration in cultured hippocampal neurons: evidence for neuritic apoptosis
- PMID: 10069579
- DOI: 10.1006/nbdi.1998.0228
Beta-amyloid induces local neurite degeneration in cultured hippocampal neurons: evidence for neuritic apoptosis
Abstract
Many apoptotic insults, including beta-amyloid, cause neuritic degeneration. The possibility that apoptotic insults act directly on neurites was investigated in experiments using compartmented cultures of hippocampal neurons. Neurites in modified Campenot chambers displayed morphological signs of degeneration, including beading or blebbing, when exposed to beta-amyloid. At short time points neurite degeneration was limited to the distal portions of neurites directly exposed to beta-amyloid. Furthermore, annexin V binding detected extracellular exposure of phosphatidylserine in portions of neurites directly exposed to apoptotic insults. Pretreatment of the cultures with zVAD-fmk blocked annexin V binding induced by beta-amyloid and concanavalin A, suggesting that caspase activity was required. Caspase activation was also visualized in neurites locally exposed to apoptotic insults. Together these results show that apoptotic insults cause local neurite degeneration which displays morphological and biochemical characteristics of apoptosis and suggest that neurite degeneration may use mechanisms common to apoptosis.
Similar articles
-
Sublethal concentrations of prion peptide PrP106-126 or the amyloid beta peptide of Alzheimer's disease activates expression of proapoptotic markers in primary cortical neurons.Neurobiol Dis. 2001 Apr;8(2):299-316. doi: 10.1006/nbdi.2001.0386. Neurobiol Dis. 2001. PMID: 11300725
-
Evidence for synaptic apoptosis.Exp Neurol. 1998 Sep;153(1):35-48. doi: 10.1006/exnr.1998.6863. Exp Neurol. 1998. PMID: 9743565
-
A beta 25-35 induces presynaptic changes in organotypic hippocampal slice cultures.Neurotoxicology. 2008 Jul;29(4):691-9. doi: 10.1016/j.neuro.2008.04.001. Epub 2008 May 27. Neurotoxicology. 2008. PMID: 18502514
-
Transport of autophagosomes in neurites of PC12 cells during serum deprivation.Autophagy. 2008 Feb;4(2):243-5. doi: 10.4161/auto.5431. Epub 2007 Dec 14. Autophagy. 2008. PMID: 18094609 Review.
-
Neurite pruning and neuronal cell death: spatial regulation of shared destruction programs.Curr Opin Neurobiol. 2013 Dec;23(6):990-6. doi: 10.1016/j.conb.2013.06.007. Epub 2013 Jul 17. Curr Opin Neurobiol. 2013. PMID: 23871216 Review.
Cited by
-
Protecting the synapse: evidence for a rational strategy to treat HIV-1 associated neurologic disease.J Neuroimmune Pharmacol. 2006 Mar;1(1):20-31. doi: 10.1007/s11481-005-9006-y. J Neuroimmune Pharmacol. 2006. PMID: 18040788 Review.
-
Protective actions of sex steroid hormones in Alzheimer's disease.Front Neuroendocrinol. 2009 Jul;30(2):239-58. doi: 10.1016/j.yfrne.2009.04.015. Epub 2009 May 7. Front Neuroendocrinol. 2009. PMID: 19427328 Free PMC article. Review.
-
Activation of sphingosine 1-phosphate receptor-1 by SEW2871 improves cognitive function in Alzheimer's disease model rats.EXCLI J. 2013 Jun 3;12:449-61. eCollection 2013. EXCLI J. 2013. PMID: 26417237 Free PMC article.
-
Ablation of specific long PDE4D isoforms increases neurite elongation and conveys protection against amyloid-β pathology.Cell Mol Life Sci. 2023 Jun 12;80(7):178. doi: 10.1007/s00018-023-04804-w. Cell Mol Life Sci. 2023. PMID: 37306762 Free PMC article.
-
The cell cycle Cdc25A tyrosine phosphatase is activated in degenerating postmitotic neurons in Alzheimer's disease.Am J Pathol. 2000 Dec;157(6):1983-90. doi: 10.1016/S0002-9440(10)64837-7. Am J Pathol. 2000. PMID: 11106571 Free PMC article.
Publication types
MeSH terms
Substances
Grants and funding
LinkOut - more resources
Full Text Sources
Other Literature Sources
