[Detection of human cytomegalovirus infection in blood donor population by the polymerase chain reaction]
- PMID: 9868113
[Detection of human cytomegalovirus infection in blood donor population by the polymerase chain reaction]
Abstract
Samples of peripheral blood from 76 different donors were detected for the presence of human cytomegalovirus (HCMV) DNA by polymerase chain reaction (PCR). The results showed that HCMVs were present in 39 out of 76 blood donors which reached an infection positive rate of 51.3%. In addition, the sensitivity and specificity on PCR were tested, it also indicated that the sensitivity can reach the level of 5fg.microliter-1. The method is simple, rapid, highly sensitive and specificity. It can be considered as a diagnostic measure in clinical detection of HCMV.
Similar articles
-
Detection of cytomegalovirus in human placental cells by polymerase chain reaction.APMIS. 2006 Nov;114(11):764-71. doi: 10.1111/j.1600-0463.2006.apm_31.x. APMIS. 2006. PMID: 17078856
-
Application of PCR in situ method for detection of human cytomegalovirus (HCMV) DNA.Acta Microbiol Pol. 2001;50(2):169-73. Acta Microbiol Pol. 2001. PMID: 11720312
-
Diagnosis of congenital cytomegalovirus infection by detection of viral DNA in urine pools.J Virol Methods. 2005 Sep;128(1-2):1-5. doi: 10.1016/j.jviromet.2005.02.018. Epub 2005 Apr 25. J Virol Methods. 2005. PMID: 16023520
-
Detection of human cytomegalovirus DNA: how, when and where?Scand J Infect Dis Suppl. 1995;99:11-5. Scand J Infect Dis Suppl. 1995. PMID: 8668932 Review.
-
[Molecular diagnosis of HCMV infection].Nihon Rinsho. 1998 Jan;56(1):121-8. Nihon Rinsho. 1998. PMID: 9465676 Review. Japanese.