Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Comparative Study
. 1998 Oct;2(4):485-93.
doi: 10.1016/s1097-2765(00)80148-3.

Formation of a novel four-helix bundle and molecular recognition sites by dimerization of a response regulator phosphotransferase

K I Varughese  1 , Madhusudan, X Z Zhou, J M Whiteley, J A Hoch
Affiliations
Free article
Comparative Study

Formation of a novel four-helix bundle and molecular recognition sites by dimerization of a response regulator phosphotransferase

K I Varughese et al. Mol Cell. 1998 Oct.
Free article

Abstract

A basis for understanding specificity of molecular recognition between phosphorelay proteins has been deduced from the 2.6 A structure of the Spo0B phosphotransferase of the phosphorelay regulating sporulation initiation. Spo0B consists of two domains: an N-terminal alpha-helical hairpin domain and a C-terminal alpha/beta domain. Two subunits of Spo0B dimerize by a parallel association of helical hairpins to form a novel four-helix bundle from which the active histidine protrudes. Docking studies show that both the monomers interact with a Spo0F molecule at the region surrounding the active site aspartate to position it for phosphotransfer. It is apparent that different surfaces of response regulators may be involved in recognition of the protein partners to which they are paired.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

Publication types

Substances

Associated data

LinkOut - more resources