MEC1-dependent phosphorylation of Rad9p in response to DNA damage
- PMID: 9734355
- DOI: 10.1016/s1097-2765(00)80128-8
MEC1-dependent phosphorylation of Rad9p in response to DNA damage
Abstract
In budding yeast, DNA damage can activate a checkpoint surveillance system controlled by the RAD9, RAD53, and MEC1 genes, resulting in a delay in cell cycle progression. Here, I report that DNA damage induces rapid and extensive phosphorylation of Rad9p in a manner that correlates directly with checkpoint activation. This response is dependent on MEC1, which encodes a member of the evolutionarily conserved ATM family of protein kinases, and on gene products of the RAD24 epistasis group, which have been implicated in the recognition and processing of DNA lesions. Since the phosphorylated form of Rad9p appears capable of interacting stably with Rad53p in vivo, this phosphorylation response likely controls checkpoint signaling by Rad9p.
Similar articles
-
Cell cycle progression in the presence of irreparable DNA damage is controlled by a Mec1- and Rad53-dependent checkpoint in budding yeast.EMBO J. 1999 Aug 16;18(16):4485-97. doi: 10.1093/emboj/18.16.4485. EMBO J. 1999. PMID: 10449414 Free PMC article.
-
Regulation of RAD53 by the ATM-like kinases MEC1 and TEL1 in yeast cell cycle checkpoint pathways.Science. 1996 Jan 19;271(5247):357-60. doi: 10.1126/science.271.5247.357. Science. 1996. PMID: 8553072
-
The budding yeast Rad9 checkpoint protein is subjected to Mec1/Tel1-dependent hyperphosphorylation and interacts with Rad53 after DNA damage.EMBO J. 1998 Oct 1;17(19):5679-88. doi: 10.1093/emboj/17.19.5679. EMBO J. 1998. PMID: 9755168 Free PMC article.
-
DNA structure dependent checkpoints as regulators of DNA repair.DNA Repair (Amst). 2002 Dec 5;1(12):983-94. doi: 10.1016/s1568-7864(02)00165-9. DNA Repair (Amst). 2002. PMID: 12531008 Review.
-
Stopped for repairs: a new role for nutrient sensing pathways?Cell Cycle. 2004 Jul;3(7):865-8. Epub 2004 Jul 17. Cell Cycle. 2004. PMID: 15190205 Review.
Cited by
-
Site-specific phosphorylation of the DNA damage response mediator rad9 by cyclin-dependent kinases regulates activation of checkpoint kinase 1.PLoS Genet. 2013 Apr;9(4):e1003310. doi: 10.1371/journal.pgen.1003310. Epub 2013 Apr 4. PLoS Genet. 2013. PMID: 23593009 Free PMC article.
-
Functions of Saccharomyces cerevisiae 14-3-3 proteins in response to DNA damage and to DNA replication stress.Genetics. 2003 Dec;165(4):1717-32. doi: 10.1093/genetics/165.4.1717. Genetics. 2003. PMID: 14704161 Free PMC article.
-
The Yeast PCNA Unloader Elg1 RFC-Like Complex Plays a Role in Eliciting the DNA Damage Checkpoint.mBio. 2019 Jun 11;10(3):e01159-19. doi: 10.1128/mBio.01159-19. mBio. 2019. PMID: 31186330 Free PMC article.
-
Activation of protein kinase Tel1 through recognition of protein-bound DNA ends.Mol Cell Biol. 2011 May;31(10):1959-71. doi: 10.1128/MCB.05157-11. Epub 2011 Mar 14. Mol Cell Biol. 2011. PMID: 21402778 Free PMC article.
-
Colocalization of sensors is sufficient to activate the DNA damage checkpoint in the absence of damage.Mol Cell. 2008 May 9;30(3):267-76. doi: 10.1016/j.molcel.2008.03.023. Mol Cell. 2008. PMID: 18471973 Free PMC article.
Publication types
MeSH terms
Substances
Grants and funding
LinkOut - more resources
Full Text Sources
Other Literature Sources
Molecular Biology Databases
Research Materials
Miscellaneous
