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. 1998 Aug;72(8):6950-5.
doi: 10.1128/JVI.72.8.6950-6955.1998.

Neutralizing antibodies in sera from macaques immunized with attenuated simian immunodeficiency virus

A J Langlois  1 , R C Desrosiers, M G Lewis, V N KewalRamani, D R Littman, J Y Zhou, K Manson, M S Wyand, D P Bolognesi, D C Montefiori
Affiliations

Neutralizing antibodies in sera from macaques immunized with attenuated simian immunodeficiency virus

A J Langlois et al. J Virol. 1998 Aug.

Abstract

Infection with attenuated simian immunodeficiency virus (SIV) in rhesus macaques has been shown to raise antibodies capable of neutralizing an animal challenge stock of primary SIVmac251 in CEMx174 cells that correlate with resistance to infection after experimental challenge with this virulent virus (M. S. Wyand, K. H. Manson, M. Garcia-Moll, D. C. Montefiori, and R. C. Desrosiers, J. Virol. 70:3724-3733, 1996). Here we show that these neutralizing antibodies are not detected in human and rhesus peripheral blood mononuclear cells (PBMC). In addition, neutralization of primary SIVmac251 in human and rhesus PBMC was rarely detected with plasma samples from a similar group of animals that had been infected either with SIVmac239Deltanef for 1.5 years or with SIVmac239Delta3 for 3.2 years, although low-level neutralization was detected in CEMx174 cells. Potent neutralization was detected in CEMx174 cells when the latter plasma samples were assessed with laboratory-adapted SIVmac251. In contrast to primary SIVmac251, laboratory-adapted SIVmac251 did not replicate in human and rhesus PBMC despite its ability to utilize CCR5, Bonzo/STRL33, and BOB/gpr15 as coreceptors for virus entry. These results illustrate the importance of virus passage history and the choice of indicator cells for making assessments of neutralizing antibodies to lentiviruses such as SIV. They also demonstrate that primary SIVmac251 is less sensitive to neutralization in human and rhesus PBMC than it is in established cell lines. Results obtained in PBMC did not support a role for neutralizing antibodies as a mechanism of protection in animals immunized with attenuated SIV and challenged with primary SIVmac251.

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Figures

FIG. 1
FIG. 1
Neutralization of primary SIVmac251 in human PBMC by plasma samples from macaques infected with either SIVmac316Δnef or SIVmac239Δ3. Each value is the average percent reduction in p27 in triplicate wells; error bars represent standard deviations. Animals in groups 1, 3, and 5 were infected with SIVmac316Δnef for 8, 20, and 79 weeks, respectively. Animals in groups 2, 4, and 6 were infected with SIVmac239Δ3 for 8, 20, and 79 weeks, respectively. +, samples positive for neutralization in CEMx174 cells; #, animals protected from challenge (7, 45).
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References

    1. Almond N, Kent K, Cranage M, Rud E, Clarke B, Stott E J. Protection by attenuated simian immunodeficiency virus in macaques against challenge with virus-infected cells. Lancet. 1995;345:1342–1344. - PubMed
    1. Berger, E. A. 1997. HIV entry and tropism: the chemokine receptor connection. AIDS 11(Suppl. A):S3–S16. - PubMed
    1. Bogers W M J M, Niphuis H, ten Haaft P, Laman J D, Koornstra W, Heeney J H. Protection from HIV-1 envelope-bearing chimeric simian immunodeficiency virus (SHIV) in rhesus macaques infected with attenuated SIV: consequences of challenge. AIDS. 1995;9:F13–F18. - PubMed
    1. Burton, D. R., and D. C. Montefiori. 1997. The antibody response in HIV-1 infection. AIDS 11(Suppl. A):S87–S98. - PubMed
    1. Chen Z, Zhou P, Ho D D, Landau N R, Marx P A. Genetically divergent strains of simian immunodeficiency virus use CCR5 as a coreceptor for entry. J Virol. 1997;71:2705–2714. - PMC - PubMed

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