Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1998 Feb 28;60(2-4):107-17.
doi: 10.1016/s0378-1135(97)00153-3.

Suppression of pseudorabies virus replication by a mutant form of immediate-early protein IE180 repressing the viral gene transcription

E Ono  1 , S Taharaguchi, S Watanabe, H Nikami, Y Shimizu, H Kida
Affiliations

Suppression of pseudorabies virus replication by a mutant form of immediate-early protein IE180 repressing the viral gene transcription

E Ono et al. Vet Microbiol. .

Abstract

A mutant form of the immediate-early (IE) protein IE180 of pseudorabies virus (PRV), dIN454-C1081 is a strong repressor of the PRV IE gene promoter. In order to assess the antiviral potential of the IE180 mutant, HeLa cells were transformed with the mutant gene and then infected with PRV and herpes simplex virus type 1 (HSV-1). The transformed cell lines showed marked resistance to PRV infection, but were susceptible to infection with HSV-1, indicating that the IE180 mutant expressed in the stable cell line specifically inhibited PRV growth. In those cells infected with PRV, transcription of the PRV IE gene was repressed. In addition, the IE180 mutant exhibited a dominant-negative property in transient expression assay. The present results indicate that the resistance of the cells to PRV infection was due to repression of the IE gene transcription by the IE 180 mutant.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

Publication types

MeSH terms

Substances

LinkOut - more resources