Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1998 Mar 15;48(3):369-72.
doi: 10.1006/geno.1997.5167.

Construction of a 1.5-Mb bacterial artificial chromosome contig in Xp11.23, a region of high gene content

K M Boycott  1 , R J Zahorchak, C G Summer, N P Boycott, V Kotak, C G Russell, N T Bech-Hansen
Affiliations

Construction of a 1.5-Mb bacterial artificial chromosome contig in Xp11.23, a region of high gene content

K M Boycott et al. Genomics. .

Abstract

To generate sequence-ready templates for the gene-rich Xp11.23 region, we have constructed a 1.5-Mb bacterial artificial chromosome (BAC) contig spanning the interval between the DNA markers OATL1 and DXS255. The contig includes 28 BACs, ranging in size from 58 to 258 kb with an average size of 135 kb, which provide 2.5-fold coverage of the region. The BAC contig was constructed based entirely on the content of 40 DNA markers from a previously established YAC contig and 11 new markers developed from BAC-end DNA sequences, 4 of which were required to close gaps in the map. There was no evidence of rearrangement, instability, or chimerism in any of the BAC clones. The BAC cloning system appears to provide robust and total physical coverage of this gene-rich region with clones that are suitable for DNA sequencing.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

Publication types

Substances

LinkOut - more resources