Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1997 Aug;9(8):1459-67.
doi: 10.1105/tpc.9.8.1459.

Cloning and expression of a gibberellin 2 beta,3 beta-hydroxylase cDNA from pumpkin endosperm

T Lange  1 , S Robatzek, A Frisse
Affiliations

Cloning and expression of a gibberellin 2 beta,3 beta-hydroxylase cDNA from pumpkin endosperm

T Lange et al. Plant Cell. 1997 Aug.

Abstract

A cDNA expression library in lambda MOSEIox derived from poly(A)+ RNA from pumpkin endosperm was screened immunologically with a polyclonal antibody raised against partially purified gibberellin (GA) 2 beta,3 beta-hydroxylase from pumpkin endosperm. A recombinant fusion protein encoded by a selected positive clone catalyzed 3 beta-hydroxylation of GA15, GA24, GA25, and GA17 and of GA12-aldehyde, GA12, GA9, and GA20, albeit less efficiently. The fusion protein also catalyzed 2 beta-hydroxylation of the C20 GAS GA25, GA13, and, as identified putatively, GA28. The full-length clone contains an open reading frame of 1041 nucleotides encoding 346 amino acid residues with a predicted molecular weight of 38,992 and pI of 7.2. Transcript levels of this gene and of the previously cloned GA 7-oxidase and 20-oxidase genes from pumpkin endosperm rose until day 2 after the start of imbibition of the mature seeds, but only at one-two hundredth to one-six thousandth of the level found in the endosperm, as determined by quantitative reverse transcriptase-polymerase chain reaction. In contrast, GA 7-oxidase, 20-oxidase, and 3 beta-hydroxylase enzyme activities were present in cell-free systems prepared from embryos of mature seeds and decreased after imbibition.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

References

    1. Annu Rev Plant Physiol Plant Mol Biol. 1997 Jun;48:431-460 - PubMed
    1. Plant Physiol. 1995 Jul;108(3):1049-57 - PubMed
    1. Proc Natl Acad Sci U S A. 1996 Sep 17;93(19):10515-8 - PubMed
    1. Proc Natl Acad Sci U S A. 1997 Jun 10;94(12):6553-8 - PubMed
    1. Plant Cell. 1995 Feb;7(2):195-201 - PubMed

Publication types

MeSH terms

Substances

LinkOut - more resources