Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1997 Jun;35(6):1450-3.
doi: 10.1128/jcm.35.6.1450-1453.1997.

Diagnosis of toxoplasmosis by joint detection of immunoglobulin A and immunoglobulin M

M Arcavi  1 , G Orfus, G Griemberg
Affiliations

Diagnosis of toxoplasmosis by joint detection of immunoglobulin A and immunoglobulin M

M Arcavi et al. J Clin Microbiol. 1997 Jun.

Abstract

An indirect immunofluorescence test with total anti-human immunoglobulin conjugate (IgG,A,M-IIF) can be used for joint detection of immunoglobulin A (IgA) and IgM antibodies, provided serum IgG is previously absorbed with anti-human IgG. To determine the validity of the IgG,A,M-IIF assay with absorbed sera, the results obtained were compared with those obtained by methods routinely used for the detection of acute-phase markers, IgA and IgM IIF and enzyme immunoassay. Accordingly, 114 serum samples were selected from patients showing titers of > or = 1:1,024 by IgG,A,M-IIF. (i) In 90 of the samples, neither IgA nor IgM was detected by any of the methods employed; (ii) the remaining 24 samples showed IgA and/or IgM. In all cases, the IgG,A,M-IIF assay with absorbed sera was positive. These comparative data support the use of IgG,A,M-IIF, performed with absorbed and unabsorbed sera simultaneously, for determining the presence of specific IgG, IgA, and IgM by employing a single technique (IIF), one conjugate (anti-IgG,A,M), and only one sample (with and without previous absorption), thus providing a useful initial tool for the diagnosis of toxoplasmosis.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

References

    1. J Clin Microbiol. 1984 Jul;20(1):133-5 - PubMed
    1. J Clin Microbiol. 1983 Jun;17(6):997-1004 - PubMed
    1. Lancet. 1985 Mar 2;1(8427):500-4 - PubMed
    1. J Clin Microbiol. 1986 Mar;23(3):576-81 - PubMed
    1. Rev Infect Dis. 1987 Sep-Oct;9(5):1055-62 - PubMed