Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1977 Nov;24(2):580-9.
doi: 10.1128/JVI.24.2.580-589.1977.

Persistent infection of cultured mammalian cells by Japanese encephalitis virus

C Schmaljohn, C D Blair

Persistent infection of cultured mammalian cells by Japanese encephalitis virus

C Schmaljohn et al. J Virol. 1977 Nov.

Abstract

Persistent infections were established by serial undiluted passage of flavivirus Japanese encephalitis virus in a line of rabbit kidney cells (MA-111). The persistently infected cells resembled uninfected cells in most respects. Low levels of infectious virions were released from a small percentage of cells, and a larger and more variable percentage was shown to possess viral antigen by fluorescent-antibody staining. Released viruses were shown to interfere with replication of wild-type Japanese encephalitis virus. Persistently infected MA-111 cells could not be superinfected with homologous wild-type Japanese encephalitis virus but could be superinfected with two heterologous viruses. Transfer of cell culture medium from persistently infected MA-111 cells to a line of African green monkey kidney cells (Vero) resulted in similar persistent infections in the latter cells. Temperature sensitivity and host-cell interferon production were not involved in establishment or maintenance of persistence. Determination of ratios of physical particles to infectious particles revealed that many defective, noninfectious viruses were present, suggesting that defective interfering particles may be responsible for persistency.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

References

    1. J Virol. 1969 Sep;4(3):244-51 - PubMed
    1. J Infect Dis. 1975 Apr;131(4):467-73 - PubMed
    1. Virology. 1975 Oct;67(2):520-33 - PubMed
    1. J Virol. 1976 Jul;19(1):90-101 - PubMed
    1. J Gen Virol. 1976 Sep;32(3):431-40 - PubMed

Publication types

LinkOut - more resources