Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1997 Feb;88(2):176-83.
doi: 10.1111/j.1349-7006.1997.tb00363.x.

Development and elongation of neurite-like outgrowth on small cell lung cancer cell lines

K Tanaka  1 , T Terasaki
Affiliations

Development and elongation of neurite-like outgrowth on small cell lung cancer cell lines

K Tanaka et al. Jpn J Cancer Res. 1997 Feb.

Abstract

One (Lu-134A) of nine human small cell lung cancer cell lines which grow as floating cell aggregates changed its morphology dramatically when cells were cultured on a coverslip coated with polyethylenimine or extracellular matrix of human lung adenocarcinoma cell line PC-9 cells. The Lu-134A cells adhered to the substrate and developed elongated cytoplasmic processes which gradually grew into long neuronal-like processes. These processes developed to a length of more than 10 times the cell body length after 20 days of culture. Addition of dibutyryl cyclic adenosine 3', 5'-monophosphate to the cells on these substrates remarkably promoted the development and elongation of the processes, which grew into a netlike arrangement. The characteristics of these elongated neuronal-like processes were studied using immunocytochemical and electron microscopical methods. The processes reacted intensely with monoclonal antibodies against beta-tubulin and microtubule-associated protein-2. The swelling portions of the distal tips of these processes reacted strongly with polyclonal antibody against synaptophysin. Neurosecretory granules and bundles of microtubules were observed within processes. These findings suggested that this human small cell lung cancer cell line (Lu-134A) differentiated into neuronal cells, and indicated that attachment of cells to a substrate is the key to the development of long neurite-like outgrowths.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

References

    1. ) Greco , F. A. and Oldman , R. K.Small–cell lung cancer . N. EngL J. Med. , 301 , 355 – 358 ( 1979. ). - PubMed
    1. ) Yoshimura , K. and Yamashita , N.Clinical statistical observation of 4,931 cases with lung cancer by histological types; results of field study in Japan . Lung Cancer 22 , 1 – 17 ( 1982. ), in Japanese .
    1. ) Vincent , R. Q. , Pickren , J. W. , Lane , W. W. , Bross , I. , Takita , H. , Houten , L. , Gutierrez , A. C. and Rzepka , T.The changing histopathology of lung cancer: a review of 1,682 cases . Cancer , 39 , 1647 – 1655 ( 1977. ). - PubMed
    1. ) Shiraosato , Y. , Nakajima , T. , Hirohashi , S. , Morinaga , S. , Terasaki , T. , Yamaguchi , K. , Saijo , N. and Suemasu , K.Biological, pathological and clinical features of small cell lung cancer . Cancer Lett. , 33 , 241 – 258 ( 1986. ). - PubMed
    1. ) Yamaguchi , K. , Abe , K. , Adachi , L , Otsubo , K. , Nagasaki , K. , Suzuki , M. , Maruno , K. , Asanuma , F. , Tsuchihashi , T. , Miyake , Y. , Yoshinaga , K. , Matsubara , H. , Imanishi , K. , Kimura , S. , Kodama , T. , Kameya , T. and Shimosato , Y.Peptide hormone production in small cell lung carcinomas with particular reference to gastrin–releasingpeptide . Jpn. J. Clin. Oncol , 16 , 235 – 241 ( 1986. ). - PubMed