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. 1996 Sep 16;15(18):5077-84.

A plant viral coat protein RNA binding consensus sequence contains a crucial arginine

P Ansel-McKinney  1 , S W Scott, M Swanson, X Ge, L Gehrke
Affiliations

A plant viral coat protein RNA binding consensus sequence contains a crucial arginine

P Ansel-McKinney et al. EMBO J. .

Erratum in

  • EMBO J 1996 Dec 16;15(24):7188-9

Abstract

A defining feature of alfalfa mosaic virus (AMV) and ilarviruses [type virus: tobacco streak virus (TSV)] is that, in addition to genomic RNAs, viral coat protein is required to establish infection in plants. AMV and TSV coat proteins, which share little primary amino acid sequence identity, are functionally interchangeable in RNA binding and initiation of infection. The lysine-rich amino-terminal RNA binding domain of the AMV coat protein lacks previously identified RNA binding motifs. Here, the AMV coat protein RNA binding domain is shown to contain a single arginine whose specific side chain and position are crucial for RNA binding. In addition, the putative RNA binding domain of two ilarvirus coat proteins, TSV and citrus variegation virus, is identified and also shown to contain a crucial arginine. AMV and ilarvirus coat protein sequence alignment centering on the key arginine revealed a new RNA binding consensus sequence. This consensus may explain in part why heterologous viral RNA-coat protein mixtures are infectious.

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References

    1. Science. 1992 Jul 3;257(5066):76-80 - PubMed
    1. Annu Rev Biophys Biomol Struct. 1992;21:95-118 - PubMed
    1. Biochemistry. 1992 Sep 29;31(38):9177-82 - PubMed
    1. Cell. 1992 Nov 13;71(4):679-90 - PubMed
    1. Proc Natl Acad Sci U S A. 1993 Feb 15;90(4):1571-5 - PubMed

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