Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1996 Jul;7(7):1043-58.
doi: 10.1091/mbc.7.7.1043.

Genes that control the fidelity of endoplasmic reticulum to Golgi transport identified as suppressors of vesicle budding mutations

M J Elrod-Erickson  1 , C A Kaiser
Affiliations
Free PMC article

Genes that control the fidelity of endoplasmic reticulum to Golgi transport identified as suppressors of vesicle budding mutations

M J Elrod-Erickson et al. Mol Biol Cell. 1996 Jul.
Free PMC article

Abstract

Although convergent evidence suggests that proteins destined for export from the endoplasmic reticulum (ER) are separated from resident ER proteins and are concentrated into transport vesicles, the proteins that regulate this process have remained largely unknown. In a screen for suppressors of mutations in the essential COPII gene SEC13, we identified three genes (BST1, BST2/EMP24, and BST3) that negatively regulate COPII vesicle formation, preventing the production of vesicles with defective or missing subunits. Mutations in these genes slow the secretion of some secretory proteins and cause the resident ER proteins Kar2p and Pdi1p to leak more rapidly from the ER, indicating that these genes are also required for proper discrimination between resident ER proteins and Golgi-bound cargo molecules. The BST1 and BST2/EMP24 genes code for integral membrane proteins that reside predominantly in the ER. Our data suggest that the BST gene products represent a novel class of ER proteins that link the regulation of vesicle coat assembly to cargo sorting.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

References

    1. EMBO J. 1995 Apr 3;14(7):1329-39 - PubMed
    1. Proc Natl Acad Sci U S A. 1995 Aug 15;92(17):8011-5 - PubMed
    1. Curr Opin Cell Biol. 1995 Aug;7(4):530-5 - PubMed
    1. J Cell Biol. 1995 Oct;131(2):311-24 - PubMed
    1. J Cell Biol. 1995 Oct;131(2):325-38 - PubMed

Publication types

MeSH terms