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. 1996 Jul-Aug;32(7):403-8.
doi: 10.1007/BF02723002.

Isolation and characterization of microvascular endothelial cells from chicken fat pads

W O Twal  1 , R M Leach
Affiliations

Isolation and characterization of microvascular endothelial cells from chicken fat pads

W O Twal et al. In Vitro Cell Dev Biol Anim. 1996 Jul-Aug.

Abstract

Microvascular endothelial cells from abdominal fat pads of 6-wk-old broiler chickens were isolated to provide an in vitro system to study their physiological functions. The isolation procedure produced clumps of 10-30 cells, which attached to culture vessels in 4 h and attained confluency in 2 wk. At confluency, cells had a cobblestone appearance but were not contact inhibited and detached from the bottom of the culture vessel 2 wk after reaching confluency. The cells internalized acetylated low density lipoproteins, a characteristic of endothelial cells. This property was used to obtain pure endothelial cell cultures using the cell sorter. When cultured over Matrigel, a reconstituted matrix, the cells aligned themselves into chordlike structures and formed branching microvessels. Cells plated on type I collagen-coated culture flasks occasionally formed chordlike structures and proliferated at a faster rate than cells plated on Matrigel. Cells cultured on laminin-coated plates were slender and had long cytoplasmic extensions; however, cells cultured on uncoated plastic had fibroblastic morphology. These properties are similar to those described for microvessel endothelial cells isolated from tissues of other species.

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References

    1. Differentiation. 1987;36(1):57-70 - PubMed
    1. J Cell Biol. 1975 Dec;67(3):863-85 - PubMed
    1. Trans Ophthalmol Soc U K. 1980 Sep;100(3):369-73 - PubMed
    1. J Histochem Cytochem. 1986 Jan;34(1):85-91 - PubMed
    1. J Biol Chem. 1981 Apr 10;256(7):3361-5 - PubMed

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