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. 1996 Apr;77(2):45-51.
doi: 10.1046/j.1365-2613.1996.962100.x.

Dengue virus-induced cytotoxin releases nitrite by spleen cells

R Mukerjee  1 , A Misra, U C Chaturvedi
Affiliations

Dengue virus-induced cytotoxin releases nitrite by spleen cells

R Mukerjee et al. Int J Exp Pathol. 1996 Apr.

Abstract

Dengue type-2 virus (DV) infection in mice induces T cells to produce a cytokine, the cytotoxic factor (CF), which induces H2-A positive macrophages to produce another cytokine, cyototoxic (CF2), which amplifies its cytotoxic effects on target cells. The present study was undertaken to investigate the production of nitrite (NO2-) by the spleen cells of mice in vitro and in vivo following inoculation of CF2. Maximum NO2- production occurred at 1 hour after inoculation of 100 micrograms CF2. Pretreatment of CF2 with anti-CF2-antisera (CF2-As) inhibited the production of NO2-. Pretreatment of the spleen cells with NG-monomethyl-L-arginine (NMA) or with arginase inhibited NO2- production. The NO2- production was diminished in a dose dependent manner by treatment of spleen cells with the Ca2+ channel blocking drug, nifedipine and Zn2+ as ZnSO4. The findings of the present study thus demonstrate that CF2 induces production of NO2- in the spleen cells in a CA(2+)-dependent manner which may be a mechanism of target cell killing.

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