doi: 10.1016/0024-3205(96)00299-8.
PACAP stimulates c-fos mRNAs in small cell lung cancer cells
Affiliations
- PMID: 8761002
- DOI: 10.1016/0024-3205(96)00299-8
Item in Clipboard
PACAP stimulates c-fos mRNAs in small cell lung cancer cells
Life Sci.
1996.
Abstract
The effects of PACAP on c-fos mRNA using small cell lung cancer (SCLC) cell was investigated. PACAP-27 (100 nM) increased c-fos mRNA 5-fold using NCI-N417 cells. The increase was concentration dependent with 0.1 nM PACAP-27 half maximally increasing c-fos mRNA. Also the increase in c-fos mRNA caused by PACAP was time dependent; being maximal after 1 hour and returning to basal values after 4 hours. PACAP-38 but not PACAP(28-38) increased c-fos mRNA. One uM PACAP(6-38), a PACAP receptor antagonist, inhibited the increase in c-fos mRNA caused by 1 nM PACAP. These data indicate that PACAP stimulates nuclear oncogene expression in SCLC cells.
Similar articles
-
Pituitary adenylate-cyclase-activating polypeptide stimulates proto-oncogene expression and activates the AP-1 (c-Fos/c-Jun) transcription factor in AR4-2J pancreatic carcinoma cells.Eur J Biochem. 1996 Dec 15;242(3):467-76. doi: 10.1111/j.1432-1033.1996.467rr.x. Eur J Biochem. 1996. PMID: 9022670
-
Bombesin stimulates c-fos and c-jun mRNAs in small cell lung cancer cells.Peptides. 1995;16(2):289-92. doi: 10.1016/0196-9781(94)00173-1. Peptides. 1995. PMID: 7784258
-
Pituitary adelylate cyclase-activating peptide is an activator of vasoactive intestinal polypeptide gene transcription in human neuroblastoma cells.Brain Res Mol Brain Res. 2000 Jun 23;79(1-2):67-76. doi: 10.1016/s0169-328x(00)00101-7. Brain Res Mol Brain Res. 2000. PMID: 10925144
-
Differential expression of pituitary adenylate cyclase-activating polypeptide/vasoactive intestinal polypeptide receptor subtypes in clonal pituitary somatotrophs and gonadotrophs.Endocrinology. 1995 May;136(5):2088-98. doi: 10.1210/endo.136.5.7720658. Endocrinology. 1995. PMID: 7720658
-
The neurotrophic activity of PACAP on rat cerebellar granule cells is associated with activation of the protein kinase A pathway and c-fos gene expression.Ann N Y Acad Sci. 1998 Dec 11;865:92-9. doi: 10.1111/j.1749-6632.1998.tb11167.x. Ann N Y Acad Sci. 1998. PMID: 9928001 Review.
Cited by
-
Protective Effects of PACAP in Peripheral Organs.Front Endocrinol (Lausanne). 2020 Jul 14;11:377. doi: 10.3389/fendo.2020.00377. eCollection 2020. Front Endocrinol (Lausanne). 2020. PMID: 32765418 Free PMC article. Review.
-
PACAP-27 tyrosine phosphorylates mitogen activated protein kinase and increases VEGF mRNAs in human lung cancer cells.Regul Pept. 2002 Nov 15;109(1-3):135-40. doi: 10.1016/s0167-0115(02)00196-9. Regul Pept. 2002. PMID: 12409225 Free PMC article.
-
PACAP immunoreactivity in human malignant tumor samples and cardiac diseases.J Mol Neurosci. 2012 Nov;48(3):667-73. doi: 10.1007/s12031-012-9815-4. Epub 2012 May 31. J Mol Neurosci. 2012. PMID: 22648511
-
Pituitary adenylate cyclase-activating polypeptide causes tyrosine phosphorylation of the epidermal growth factor receptor in lung cancer cells.J Pharmacol Exp Ther. 2012 Jun;341(3):873-81. doi: 10.1124/jpet.111.190033. Epub 2012 Mar 2. J Pharmacol Exp Ther. 2012. PMID: 22389426 Free PMC article.
-
Pituitary adenylate cyclase-activating polypeptide causes increased tyrosine phosphorylation of focal adhesion kinase and paxillin.J Mol Neurosci. 2012 Jan;46(1):68-74. doi: 10.1007/s12031-011-9639-7. Epub 2011 Sep 6. J Mol Neurosci. 2012. PMID: 21898124
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
