doi: 10.1007/BF00338691.
Kinetics of Mu DNA synthesis
- PMID: 876022
- DOI: 10.1007/BF00338691
Item in Clipboard
Kinetics of Mu DNA synthesis
Mol Gen Genet.
.
Abstract
Mu specific DNA synthesis starts at 10 min after infection. All essentail amber mutants of Mu were tested for the ability to replicate in a non permissive host. Except for the amber mutants A and B, which were already known to be blocked in Mu DNA synthesis (Wijffelman et al., 1974), all the other mutants showed normal Mu DNA replication. Using mitomycin C-treated cells Mu DNA synthesis was found to start at about 20 min after induction. However using the much more sensitive method of DNA-RNA hybridization, it was found that the DNA synthesis starts already at 10 min after induction, and that at 20 min after induction about 7 copies of the Mu DNA are present per cell.
Similar articles
-
Process of infection with bacteriophage phi chi 174. XL. Viral DNA replication of phi chi 174 mutants blocked in progeny single-stranded DNA synthesis.J Virol. 1976 Apr;18(1):218-26. doi: 10.1128/JVI.18.1.218-226.1976. J Virol. 1976. PMID: 1255871 Free PMC article.
-
Inactivation of DNA-binding activity of repressor in extracts of lambda-lysogen treated with mitomycin C.Mol Gen Genet. 1973 Nov 2;126(2):103-10. doi: 10.1007/BF00330987. Mol Gen Genet. 1973. PMID: 4591375 No abstract available.
-
In vivo production of an RNA-DNA copolymer after infection of Escherichia coli by bacteriophage T4.Proc Natl Acad Sci U S A. 1972 Nov;69(11):3165-9. doi: 10.1073/pnas.69.11.3165. Proc Natl Acad Sci U S A. 1972. PMID: 4564205 Free PMC article.
-
Lysogeny: viral repression and site-specific recombination.Annu Rev Biochem. 1971;40:827-54. doi: 10.1146/annurev.bi.40.070171.004143. Annu Rev Biochem. 1971. PMID: 4941241 Review. No abstract available.
-
T4 and the rolling circle model of replication.Annu Rev Genet. 1973;7:325-41. doi: 10.1146/annurev.ge.07.120173.001545. Annu Rev Genet. 1973. PMID: 4593307 Review. No abstract available.
Cited by
-
Bacteriophage Mu DNA replication is stimulated by non-essential early functions.Mol Gen Genet. 1982;186(1):135-9. doi: 10.1007/BF00422925. Mol Gen Genet. 1982. PMID: 7050622
-
Effects of prophage Mu induction on expression of adjacent host genes.Mol Biol Rep. 1980 Dec 31;6(4):229-34. doi: 10.1007/BF00777530. Mol Biol Rep. 1980. PMID: 6451799
-
Identification of a positive regulator of the Mu middle operon.J Bacteriol. 1990 Dec;172(12):6641-50. doi: 10.1128/jb.172.12.6641-6650.1990. J Bacteriol. 1990. PMID: 2147678 Free PMC article.
-
Stimulation of deletions in the Escherichia coli chromosome by partially induced Mucts62 prophages.J Bacteriol. 1978 Nov;136(2):477-83. doi: 10.1128/jb.136.2.477-483.1978. J Bacteriol. 1978. PMID: 361710 Free PMC article.
-
Escherichia coli K-12 gyrB gene product is involved in the lethal effect of the ligts2 mutant of bacteriophage Mu.J Bacteriol. 1984 Feb;157(2):665-8. doi: 10.1128/jb.157.2.665-668.1984. J Bacteriol. 1984. PMID: 6319373 Free PMC article.
References
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Research Materials
