An in vitro model of the rat dorsal root entry zone reveals developmental changes in the extent of sensory axon growth into the spinal cord
- PMID: 8726103
- DOI: 10.1006/mcne.1996.0015
An in vitro model of the rat dorsal root entry zone reveals developmental changes in the extent of sensory axon growth into the spinal cord
Abstract
The dorsal root entry zone (DREZ) forms the junction between the dorsal roots of the peripheral nervous system and the spinal cord. In rats older than 1 week, lesioned primary sensory axons regenerate within the dorsal roots but stop at the DREZ, and are thus unable to reconnect with the spinal cord. To analyze the causes of this failure, we have developed a culture model of the interaction of sensory axon growth cones with the intact DREZ, whereby dissociated dorsal root ganglion neurons from rats of various ages are grown on longitudinal cryosections of rat spinal cord, incorporating the DREZ and attached dorsal roots, from neonatal, 1-week-old (P6), or adult animals. Neurites of all ages grew along the roots to the DREZ, where their ability to cross into the spinal cord depended on both their age and that of the spinal cord substrate. Neurites from neonatal neurons failed to cross either the P6 or adult DREZ, but a substantial proportion crossed the immature neonatal DREZ. Early embryonic neurites exhibited substantial crossing on both immature and adult DREZ. These findings strongly suggest that soon after birth, the normal mammalian DREZ acquires growth inhibitory activity that is recognized by the axons of postnatal but not early embryonic sensory neurons.
Similar articles
-
Behaviour of DRG sensory neurites at the intact and injured adult rat dorsal root entry zone: postnatal neurites become paralysed, whilst injury improves the growth of embryonic neurites.Glia. 1999 Jun;26(4):309-23. Glia. 1999. PMID: 10383050
-
Border controls at the mammalian spinal cord: late-surviving neural crest boundary cap cells at dorsal root entry sites may regulate sensory afferent ingrowth and entry zone morphogenesis.Mol Cell Neurosci. 1997;9(5-6):381-96. doi: 10.1006/mcne.1997.0647. Mol Cell Neurosci. 1997. PMID: 9361276
-
Nerve growth factor promotes regeneration of sensory axons into adult rat spinal cord.Exp Neurol. 1996 Aug;140(2):218-29. doi: 10.1006/exnr.1996.0131. Exp Neurol. 1996. PMID: 8690064
-
[Strategies to repair lost sensory connections to the spinal cord].Mol Biol (Mosk). 2008 Sep-Oct;42(5):820-9. Mol Biol (Mosk). 2008. PMID: 18988531 Review. Russian.
-
Chemorepulsion and cell adhesion molecules in patterning initial trajectories of sensory axons.Neurosci Res. 2005 Apr;51(4):337-47. doi: 10.1016/j.neures.2005.01.007. Neurosci Res. 2005. PMID: 15740797 Review.
Cited by
-
The transitional zone and CNS regeneration.J Anat. 1999 Feb;194(Pt 2)(Pt 2):161-82. doi: 10.1046/j.1469-7580.1999.19420161.x. J Anat. 1999. Corrected and republished in: J Anat. 2000 Jan;196 ( Pt 1):137-58. PMID: 10337949 Free PMC article. Corrected and republished. Review.
-
Two-tiered inhibition of axon regeneration at the dorsal root entry zone.J Neurosci. 2001 Apr 15;21(8):2651-60. doi: 10.1523/JNEUROSCI.21-08-02651.2001. J Neurosci. 2001. PMID: 11306618 Free PMC article.
-
Neuronal matrix metalloproteinase-2 degrades and inactivates a neurite-inhibiting chondroitin sulfate proteoglycan.J Neurosci. 1998 Jul 15;18(14):5203-11. doi: 10.1523/JNEUROSCI.18-14-05203.1998. J Neurosci. 1998. PMID: 9651203 Free PMC article.
-
Engineering an integrated cellular interface in three-dimensional hydrogel cultures permits monitoring of reciprocal astrocyte and neuronal responses.Tissue Eng Part C Methods. 2012 Jul;18(7):526-36. doi: 10.1089/ten.TEC.2011.0587. Epub 2012 Feb 24. Tissue Eng Part C Methods. 2012. PMID: 22235832 Free PMC article.
-
Modulating Sema3A signal with a L1 mimetic peptide is not sufficient to promote motor recovery and axon regeneration after spinal cord injury.Mol Cell Neurosci. 2008 Feb;37(2):222-35. doi: 10.1016/j.mcn.2007.09.009. Epub 2007 Oct 3. Mol Cell Neurosci. 2008. PMID: 17997325 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Research Materials
