One-step purification of recombinant proteins with the 6xHis tag and Ni-NTA resin
- PMID: 8713899
- DOI: 10.1385/0-89603-402-X:491
One-step purification of recombinant proteins with the 6xHis tag and Ni-NTA resin
Similar articles
-
One-step purification of recombinant proteins with the 6xHis tag and Ni-NTA resin.Mol Biotechnol. 1995 Dec;4(3):247-58. doi: 10.1007/BF02779018. Mol Biotechnol. 1995. PMID: 8680931
-
6xHis-Ni-NTA chromatography as a superior technique in recombinant protein expression/purification.Methods Mol Biol. 1994;31:371-87. doi: 10.1385/0-89603-258-2:371. Methods Mol Biol. 1994. PMID: 7921034 Review. No abstract available.
-
Double-hexahistidine tag with high-affinity binding for protein immobilization, purification, and detection on ni-nitrilotriacetic acid surfaces.Anal Chem. 2006 May 1;78(9):3072-9. doi: 10.1021/ac060184l. Anal Chem. 2006. PMID: 16642995
-
Ni-NTA-gold clusters target His-tagged proteins.J Struct Biol. 1999 Sep;127(2):185-98. doi: 10.1006/jsbi.1999.4149. J Struct Biol. 1999. PMID: 10527908
-
Expression and purification of soluble adenylyl cyclase from Escherichia coli.Methods Mol Biol. 2004;237:39-53. doi: 10.1385/1-59259-430-1:39. Methods Mol Biol. 2004. PMID: 14501037 Review.
Cited by
-
Characterization of camel nanobodies specific for superfolder GFP fusion proteins.Mol Biol Rep. 2014 Oct;41(10):6887-98. doi: 10.1007/s11033-014-3575-x. Epub 2014 Aug 2. Mol Biol Rep. 2014. PMID: 25085037
-
Identifying Protein Interactions with Histone Peptides Using Bio-layer Interferometry.Bio Protoc. 2018 Sep 20;8(18):e3012. doi: 10.21769/BioProtoc.3012. eCollection 2018 Sep 20. Bio Protoc. 2018. PMID: 34395802 Free PMC article.
-
Transgenic expression and purification of myosin isoforms using the Drosophila melanogaster indirect flight muscle system.Methods. 2012 Jan;56(1):25-32. doi: 10.1016/j.ymeth.2011.12.002. Epub 2011 Dec 8. Methods. 2012. PMID: 22178692 Free PMC article.
-
Identification and molecular characterization of a psychrophilic GH1 β-glucosidase from the subtropical soil microorganism Exiguobacterium sp. GXG2.AMB Express. 2019 Oct 1;9(1):159. doi: 10.1186/s13568-019-0873-7. AMB Express. 2019. PMID: 31576505 Free PMC article.
-
Cloning, expression, and one-step purification/immobilization of two carbohydrate-binding module-tagged alcohol dehydrogenases.J Biol Eng. 2022 Jun 28;16(1):16. doi: 10.1186/s13036-022-00295-8. J Biol Eng. 2022. PMID: 35765016 Free PMC article.
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources