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. 1995 Dec;4(3):247-58.
doi: 10.1007/BF02779018.

One-step purification of recombinant proteins with the 6xHis tag and Ni-NTA resin

J Crowe  1 , B S Masone, J Ribbe
Affiliations

One-step purification of recombinant proteins with the 6xHis tag and Ni-NTA resin

J Crowe et al. Mol Biotechnol. 1995 Dec.

Abstract

The 6xHis/Ni-NTA system allows rapid and efficient affinity purification of recombinant proteins from virtually any expression system. Protocols and tips for purification under both native and denaturing conditions are provided, as well as a rapid spin procedure for protein minipreps.

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