doi: 10.1093/nar/4.3.723.
Studies of gene control regions. III. Binding of synthetic and modified synthetic lac operator DNAs to lactose repressor
- PMID: 866187
- PMCID: PMC342475
- DOI: 10.1093/nar/4.3.723
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Studies of gene control regions. III. Binding of synthetic and modified synthetic lac operator DNAs to lactose repressor
Nucleic Acids Res.
1977 Mar.
Free PMC article
Abstract
Chemically synthesized lactose operator DNA was tested for binding with lactose repressor protein. These operator DNAs were found to (1) bind specifically to lactose SQ repressor as measured by release of binding with the inducing ligand isopropyl-beta-D-thiogalactoside, (2) have dissociation half-lives of 37 seconds (21 base-paired duplex) and 46 seconds (26 base-paired duplex) and (3) have dissociation half-lives with x86 repressor of 9 minutes (21 base-paired duplex) and 18 minutes (26 base paired duplex). Modified operators containing 5-bromodeoxyuridine and deoxyuridine at specific sites were also prepared. These analogs bound both repressors about as tightly as the wild type sequence.
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