Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1996 Jun;58(6):1239-46.

Testing the feasibility of DNA typing for human identification by PCR and an oligonucleotide ligation assay

C Delahunty  1 , W Ankener, Q Deng, J Eng, D A Nickerson
Affiliations

Testing the feasibility of DNA typing for human identification by PCR and an oligonucleotide ligation assay

C Delahunty et al. Am J Hum Genet. 1996 Jun.

Abstract

The use of DNA typing in human genome analysis is increasing and finding widespread application in the area of forensic and paternity testing. In this report, we explore the feasibility of typing single nucleotide polymorphisms (SNPs) by using a semiautomated method for analyzing human DNA samples. In this approach, PCR is used to amplify segments of human DNA containing a common SNP. Allelic nucleotides in the amplified product are then typed by a colorimetric implementation of the oligonucleotide ligation assay (OLA). The results of the combined assay, PCR/OLA, are read directly by a spectrophotometer; the absorbances are compiled; and the genotypes are automatically determined. A panel of 20 markers has been developed for DNA typing and has been tested using a sample panel from the CEPH pedigrees (CEPH parents). The results of this typing, as well as the potential to apply this method to larger populations, are discussed.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

References

    1. Nucleic Acids Res. 1994 Oct 11;22(20):4167-75 - PubMed
    1. Proc Natl Acad Sci U S A. 1994 May 24;91(11):5022-6 - PubMed
    1. Electrophoresis. 1994 Jul;15(7):890-5 - PubMed
    1. Genomics. 1994 Sep 1;23(1):138-44 - PubMed
    1. Heredity (Edinb). 1995 Mar;74 ( Pt 3):274-85 - PubMed

Publication types

LinkOut - more resources