Competition between globin messenger ribonucleic acids for a discriminating initiation factor
- PMID: 856800
Competition between globin messenger ribonucleic acids for a discriminating initiation factor
Abstract
Translation of messenger ribonucleic acids for alpha- and beta-globin chains was analyzed in an mRNA-dependent fractionated protein-synthesizing system derived from rabbit reticulocytes. The alpha/beta chain synthesis ratio is highly dependent on the concentration of unfractionated globin mRNAs; the ratio is 1.5 at low mRNA concentration and declines to 0.03 at a high concentration. Several lines of evidence support the conclusion that this effect is caused by competitive binding of the messengers to an initiation factor which preferentially associates with the beta-mRNA. Such a discriminating factor is present in the 0.5 M KCl wash fraction from ribosomes and it elutes from a diethylaminoethyl-cellulose column between 0.10 and 0.21 M KCl. Studies using purified preparations of initiation factors suggest that IF-M3 and IF-M4 may act synergistically to produce the activity of the discriminating initiation factor. Although the discriminating factor is required for translation of both messengers, its apparent binding constant to beta-mRNA is 50 times larger than to alpha-mRNA. The concentration of discriminating factor-mRNA complex does not limit the overall rate of protein synthesis in this cell-free system. Nevertheless, the relative effectiveness of different messengers is determined by the relative concentrations of their complexes with the discriminating factor.
Similar articles
-
Requirement for homologous rabbit reticulocyte initiation factor 3 for initiation of alpha- and beta-globin mRNA translation in a crude protozoal cell-free system.J Biol Chem. 1976 Sep 25;251(18):5718-25. J Biol Chem. 1976. PMID: 965388
-
Competition between alpha- and beta-globin messenger ribonucleic acids for eucaryotic initiation factor 2.Biochemistry. 1979 Jun 26;18(13):2847-54. doi: 10.1021/bi00580a027. Biochemistry. 1979. PMID: 476057 No abstract available.
-
Relationship between the discriminating factor and the globin messenger RNA in differential alpha and beta globin synthesis.FEBS Lett. 1976 Dec 15;72(1):43-7. doi: 10.1016/0014-5793(76)80809-5. FEBS Lett. 1976. PMID: 1001468 No abstract available.
-
Importance of initiation factor preparations in the translation of reovirus and globin mRNAs lacking a 5'-terminal 7-methylguanosine.J Biol Chem. 1977 Dec 10;252(23):8489-97. J Biol Chem. 1977. PMID: 925007
-
Translational control of protein synthesis: the early years.J Biol Chem. 2012 Oct 19;287(43):36528-35. doi: 10.1074/jbc.X112.420356. Epub 2012 Sep 17. J Biol Chem. 2012. PMID: 22988251 Free PMC article. Review.
Cited by
-
Eukaryotic mRNA cap binding protein: purification by affinity chromatography on sepharose-coupled m7GDP.Proc Natl Acad Sci U S A. 1979 Sep;76(9):4345-9. doi: 10.1073/pnas.76.9.4345. Proc Natl Acad Sci U S A. 1979. PMID: 291969 Free PMC article.
-
In vitro transcription and translational efficiency of chimeric SP6 messenger RNAs devoid of 5' vector nucleotides.Nucleic Acids Res. 1988 May 25;16(10):4483-98. doi: 10.1093/nar/16.10.4483. Nucleic Acids Res. 1988. PMID: 3260027 Free PMC article.
-
Contribution of polyadenylate sequences to the translational efficiency of globin messenger RNAs.Biochem J. 1987 Sep 15;246(3):575-81. doi: 10.1042/bj2460575. Biochem J. 1987. PMID: 3689323 Free PMC article.
-
Increased rates of polypeptide chain elongation in placental explants from human diabetics.Proc Natl Acad Sci U S A. 1984 Mar;81(5):1366-70. doi: 10.1073/pnas.81.5.1366. Proc Natl Acad Sci U S A. 1984. PMID: 6584885 Free PMC article.
-
The function of proteins that interact with mRNA.Mol Cell Biochem. 1987 Mar;74(1):5-15. doi: 10.1007/BF00221907. Mol Cell Biochem. 1987. PMID: 3295516 Review.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Miscellaneous
