Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1993 Mar 25;21(6):1389-95.
doi: 10.1093/nar/21.6.1389.

The secondary structure of the 5'-noncoding region of beet necrotic yellow vein virus RNA 3: evidence for a role in viral RNA replication

D Gilmer  1 , C Allmang, C Ehresmann, H Guilley, K Richards, G Jonard, B Ehresmann
Affiliations
Free PMC article

The secondary structure of the 5'-noncoding region of beet necrotic yellow vein virus RNA 3: evidence for a role in viral RNA replication

D Gilmer et al. Nucleic Acids Res. .
Free PMC article

Abstract

Secondary structure-sensitive chemical and enzymatic probes have been used to produce a model for the folding of the first 312 residues of the long 5'-noncoding region of beet necrotic yellow vein virus RNA 3. The structure consists of two major domains, one of which includes long distance base-pairing interactions between two short sequence elements (Box I and Box II) situated between positions 237 and 292 and complementary elements (Box I' and II') near the 5'-terminus. Previous studies have shown that base pairing between these sequence elements (in either the plus-strand or minus-strand RNA) is important for RNA 3 accumulation during infection. RNA 3 transcripts were produced containing mutations which preferentially disrupted Box II-II' base pairing in either the plus- or minus-strand. In infection experiments, transcripts with mutations which disrupted the Box II-II' interaction in the plus-strand structure replicated less efficiently than mutants in which the Box II-II' interaction was disrupted in the minus-strand. These findings indicate that the complex 5'-proximal plus-strand structure to which the Box II-II' interaction contributes comprises at least part of the promoter for plus-strand RNA synthesis.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

References

    1. Virology. 1989 Oct;172(2):415-27 - PubMed
    1. Virology. 1992 Sep;190(1):55-67 - PubMed
    1. J Biomol Struct Dyn. 1986 Oct;4(2):193-203 - PubMed
    1. Nature. 1990 Aug 16;346(6285):680-2 - PubMed
    1. Virology. 1990 Sep;178(1):152-60 - PubMed