Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1993 May 1;90(9):4304-8.
doi: 10.1073/pnas.90.9.4304.

General involvement of hypoxia-inducible factor 1 in transcriptional response to hypoxia

G L Wang  1 , G L Semenza
Affiliations

General involvement of hypoxia-inducible factor 1 in transcriptional response to hypoxia

G L Wang et al. Proc Natl Acad Sci U S A. .

Abstract

Transcription of the human erythropoietin (EPO) gene is activated in Hep3B cells exposed to hypoxia. Hypoxia-inducible factor 1 (HIF-1) is a nuclear factor whose DNA binding activity is induced by hypoxia in Hep3B cells, and HIF-1 binds at a site in the EPO gene enhancer that is required for hypoxic activation of transcription. In this paper, we demonstrate that HIF-1 DNA binding activity is also induced by hypoxia in a variety of mammalian cell lines in which the EPO gene is not transcribed. The composition of the HIF-1 DNA binding complex and its isolated DNA binding subunit and the mechanism of HIF-1 activation appear to be similar or identical in EPO-producing and non-EPO-producing cells. Transcription of reporter genes containing the EPO gene enhancer is induced by hypoxia in non-EPO-producing cells and mutations that eliminate HIF-1 binding eliminate inducibility. These results provide evidence that HIF-1 and its recognition sequence are common components of a general mammalian cellular response to hypoxia.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

References

    1. Nature. 1970 Aug 15;227(5259):680-5 - PubMed
    1. J Clin Invest. 1990 Aug;86(2):670-4 - PubMed
    1. Cell. 1988 Aug 26;54(5):671-83 - PubMed
    1. Science. 1988 Dec 9;242(4884):1412-5 - PubMed
    1. Proc Natl Acad Sci U S A. 1989 Apr;86(7):2301-5 - PubMed

Publication types

MeSH terms

LinkOut - more resources