Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1993 Feb;21(3):475-85.
doi: 10.1007/BF00028805.

Protein phosphatases in higher plants: multiplicity of type 2A phosphatases in Arabidopsis thaliana

J Ariño  1 , E Pérez-Callejón, N Cunillera, M Camps, F Posas, A Ferrer
Affiliations

Protein phosphatases in higher plants: multiplicity of type 2A phosphatases in Arabidopsis thaliana

J Ariño et al. Plant Mol Biol. 1993 Feb.

Abstract

Two DNA fragments, AP-1 and AP-2, encoding amino acid sequences closely related to Ser/Thr protein phosphatases were amplified from Arabidopsis thaliana genomic DNA. Fragment AP-1 was used to screen A. thaliana cDNA libraries and several positive clones were isolated. Clones EP8a and EP14a were sequenced and found to encode almost identical proteins (97% identity). Both proteins are 306 amino acids in length and are very similar (79-80% identity) to the mammalian isotypes of the catalytic subunit of protein phosphatase 2A. Therefore, they have been designated PP2A-1 and PP2A-2. A third cDNA clone, EP7, was isolated and sequenced. The polypeptide encoded (308 amino acids, lacking the initial Met codon) is 80% identical with human phosphatases 2A and was named PP2A-3. The PP2A-3 protein is extremely similar (95% identity) to the predicted protein from a cDNA clone previously found in Brassica napus. Southern blot analysis of genomic DNA using AP-1 and AP-2 probes, as well as probes derived from clones EP7, EP8a and EP14a strongly indicates that at least 6 genes closely related to type 2A phosphatases are present in the genome of A. thaliana. Northern blot analysis using the same set of probes demonstrates that, at the seedling stage, the mRNA levels for PP2A-1, PP2A-3 and the gene containing the AP-1 sequence are much higher than those of PP2A-2 and AP-2. These results demonstrate that a multiplicity of type 2A phosphatases might be differentially expressed in higher plants.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

References

    1. Science. 1983 Jul 22;221(4608):331-8 - PubMed
    1. Biochemistry. 1991 Jan 8;30(1):89-97 - PubMed
    1. EMBO J. 1990 Dec;9(13):4339-46 - PubMed
    1. EMBO J. 1987 Jan;6(1):43-8 - PubMed
    1. Biochem J. 1988 Apr 15;251(2):357-63 - PubMed

Publication types

LinkOut - more resources